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Study Information
Study Attribute Value
CEBS Accession Number: 002-02577-0001-0000-4
Chemical Name: Nalidixic acid
CASRN: 389-08-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 404155_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/05/1984    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 410 0.393 8.20 27.5 0.000
Negative (Not Specified) 
0         
 50 1043 379 0.363 7.58 27.5 -7.472
Test Chemical Nalidixic acid 
5         
 50 1039 431 0.415 8.62 27.5 5.628 
16         
 50 1039 488 0.470 9.76 27.5 19.597 
50         
 50 1041 426 0.409 8.52 27.5 4.202 
160         
 50 1042 502 0.482 10.04 27.5 22.674
Positive Control Mitomycin-C 
 0.0E-4    
 50 1045 539 0.516 10.78 27.5 31.338
Mitomycin-C 
 0.001     
 10 208 164 0.788 16.40 27.5 100.769
Trend: 2.684        
Probability: 0.004        

Trial #: 2    Activation: No Activation    Date: 08/07/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 450 0.429 9.00 26.5 0.000
Test Chemical Nalidixic acid 
50         
 50 1048 476 0.454 9.52 26.5 5.778 
75         
 50 1049 512 0.488 10.24 26.5 13.669 
100         
 50 1049 501 0.478 10.02 26.5 11.227 
150         
 50 1047 509 0.486 10.18 26.5 13.219
Positive Control Mitomycin-C 
 0.0E-4    
 50 1052 644 0.612 12.88 26.5 42.567
Mitomycin-C 
 0.01      
 10 210 580 2.762 58.00 26.5 543.217
Trend: 2.088        
Probability: 0.018        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/05/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 467 0.445 9.34 26.0 0.000
Negative (Not Specified) 
0         
 50 1043 444 0.426 8.88 26.0 -4.287
Test Chemical Nalidixic acid 
5         
 50 1051 458 0.436 9.16 26.0 -2.021 
16         
 50 1053 423 0.402 8.46 26.0 -9.680 
50         
 50 1051 437 0.416 8.74 26.0 -6.513 
160         
 50 1050 505 0.481 10.10 26.0 8.137
Positive Control Cyclophosphamide 
 0.3       
 50 1044 607 0.581 12.14 26.0 30.726
Cyclophosphamide 
2         
 10 210 381 1.814 38.10 26.0 307.923
Trend: 0.812        
Probability: 0.208        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/07/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 418 0.399 8.36 26.0 0.000
Test Chemical Nalidixic acid 
50         
 50 1046 425 0.406 8.50 26.0 1.869 
75         
 50 1049 434 0.414 8.68 26.0 3.729 
100         
 50 1049 404 0.385 8.08 26.0 -3.441 
150         
 50 1046 396 0.379 7.92 26.0 -5.082
Positive Control Cyclophosphamide 
 0.3       
 50 1050 605 0.576 12.10 26.0 44.461
Cyclophosphamide 
2         
 10 209 414 1.981 41.40 26.0 396.637
Trend: -0.962        
Probability: 0.832