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Study Information
Study Attribute Value
CEBS Accession Number: 002-02577-0009-0000-2
Chemical Name: Nalidixic acid
CASRN: 389-08-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 803923
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 97 115 96 33 38
    91 105 77 28
    92 122 128 46
    80 58 107 44
Test Chemical Nalidixic acid 
30         
 51 78 47 31 31
    70 94 62 29
    72 92 73 34
  
40         
 65 76 74 38 37
    81 121 102 42
    45 108 42 31
  
50         
 84 58 92 37 37
    89 64 98 37
    78 72 90 38
  
80         
 76 81 73 32 28
    84 72 63 25
    86 78 72 28
  
100         
 77 97 93 40 33
    96 127 83 29
    60 73 55 31
Positive Control Methyl Methane Sulfonate 
5         
 372*
    59 58 644 362
    48 48 555 383

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 118 112 48 14 29
    121r 119 104 29
    74# 89 81 36
    99 99 112 38
Test Chemical Nalidixic acid 
30         
 108 93 114 35 35
    102 81 99 32
    87# 86 95 36
  
40         
 102 80 108 35 43
    95 123 142 50
    87 46 112 43
  
50         
 118# 100 93 26 32
    91 86 97 36
    98 96 97 33
  
60         
 94 94 96 34 38
    101 84 122 40
    93 77 107 39
  
80         
 80 104 92 39 38
    92 69 104 38
    105 102 123 39
  
100         
 127r 88 103 27 37
    90 82 107 40
    84 78 85 34
Positive Control Methyl Methane Sulfonate 
5         
 87 64 493 189 196*
    85 58 477 188
    70 60 443 210

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 140r 121 175 42 62
    120 84 182 51
    131r 127 209 53
    118 116 262 74
Test Chemical Nalidixic acid 
30         
 100 37 240 80 78
    88 41 186 71
    112 80 281 84
  
40         
 103 73 212 69 73
    99 31 229 77
    129r 132 159 41
  
50         
 134r 109 153 38 67
    120 60 189 53
    98# 27 237 81
  
60         
 126r 109 204 54
    132r 97 204 52
    141r 119 195 46
  
80         
 123r 109 140 38
    130r 101 262 67
    103 49 303 98
  
100         
 132r 129 209 53
    130r 101 211 54
    124r 115 226 61
Positive Control 3-Methylcholanthrene 
2.5       
 115 33 585 170 216*
    116 33 753 216
    110 28 859 261

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 105 84 30 20
    109 99 52 16
    103 95 50 16
    102 100 53 17
Test Chemical Nalidixic acid 
30         
 107# 97 52 16 19
    111# 85 44 13
    96# 82 82 28
  
40         
 82 74 60 25 19
    107 91 50 16
    100 82 49 16
  
50         
 109 89 40 12 17
    75 69 32 14
    103 81 72 23
  
60         
 127r 93 78 20
    157r 111 74 16
    113 69 68 20
  
80         
 113 97 85 25 28
    96# 86 83 29
    114 79 98 29
  
100         
 100 77 85 28 24
    131r 89 97 25
    106 83 65 20
Positive Control 3-Methylcholanthrene 
2.5       
 44 12 603 459 409*
    66 27 602 303
    37 12 521 465

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 124r 98 122 33 24
    105 108 84 27
    132r 112 92 23
    104 92 67 21
Test Chemical Nalidixic acid 
30         
 98 83 82 28 29
    97 70 106 36
    105 96 69 22
  
40         
 100 89 49 16 27
    80 83 85 35
    111 81 102 31
  
50         
 108 91 150 46 35
    86 82 95 37
    90 92 61 23
  
60         
 114 99 93 27 27
    105 106 85 27
    122r 74 90 25
  
80         
 92 94 64 23 23
    115 83 83 24
    105 72 71 23
  
100         
 135r 87 127 31 23
    111 94 81 24
    100 87 65 22
Positive Control 3-Methylcholanthrene 
2.5       
 66 13 761 385 441*
    61 14 814 447
    50 13 739 489

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination