Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-02586-0006-0000-9
Chemical Name: Nickel sulfate hexahydrate
CASRN: 10101-97-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 411811
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 51# 107 117 76 61
    59 76 112 63
    70 117 94 45
Test Chemical Nickel sulfate hexahydrate 
300         
 89# 148 120 45
  
400         
 81 118 120 49 51
    75# 132 117 52
  
500         
 72# 90 130.5 61 72
    71 105 178 83
  
600         
 65 19 235 120 99*
    76 33 180 79
  
700         
 45# 6 312 233 214*
    47 8 274 194
  
800         
   
Positive Control Methyl Methane Sulfonate 
15         
 30# 47 265.5 297 265*
    45# 50 312 234

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 110 101 168 51 50
    87 98 145 56
    111 103 129 39
    94 98 154 55
Test Chemical Nickel sulfate hexahydrate 
400         
 71 37 165 78 72
    95 40 190 67
  
500         
 86 39 173 67 90*
    61 25 205 112
  
600         
 47 12 262 187 165*
    65 16 279 143
  
700         
 30 5 365 408 287*
    73 8 362 166
  
800         
 51 5 292 192 209*
    43 5 293 226
  
900         
   
Positive Control Methyl Methane Sulfonate 
15         
 61 32 227 125 159*
    55 30 317 193

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination