Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02586-0006-0000-9
Chemical Name:	Nickel sulfate hexahydrate
CASRN:	10101-97-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	411811
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	51#	107	117	76	61
			59	76	112	63
			70	117	94	45
Test Chemical	Nickel sulfate hexahydrate	300	89#	148	120	45
		400	81	118	120	49	51
			75#	132	117	52	51
		500	72#	90	130.5	61	72
			71	105	178	83	72
		600	65	19	235	120	99*
			76	33	180	79	99*
		700	45#	6	312	233	214*
			47	8	274	194	214*
		800

Positive Control	Methyl Methane Sulfonate	15	30#	47	265.5	297	265*
Positive Control			45#	50	312	234	265*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	110	101	168	51	50
			87	98	145	56
			111	103	129	39
			94	98	154	55
Test Chemical	Nickel sulfate hexahydrate	400	71	37	165	78	72
			95	40	190	67	72
		500	86	39	173	67	90*
			61	25	205	112	90*
		600	47	12	262	187	165*
			65	16	279	143	165*
		700	30	5	365	408	287*
			73	8	362	166	287*
		800	51	5	292	192	209*
			43	5	293	226	209*
		900

Positive Control	Methyl Methane Sulfonate	15	61	32	227	125	159*
Positive Control			55	30	317	193	159*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination