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Study Information
Study Attribute Value
CEBS Accession Number: 002-02594-0001-0000-3
Chemical Name: Nitrilotriacetic acid trisodium monohydrate
CASRN: 18662-53-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 696583_SCE
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
No Activation  3  Positive 
No Activation  4  Positive 
No Activation  5  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 02/07/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 382 0.366 7.64 27.0 0.000
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
16         
 50 1043 397 0.381 7.94 27.0 4.026 
50         
 50 1041 447 0.429 8.94 27.0 17.353 
160         
 50 1037 428 0.413 8.56 27.0 12.798 
500         
 50 1044 537 0.514 10.74 27.0 40.576
Positive Control Mitomycin-C 
 0.001     
 50 1043 659 0.632 13.18 27.0 72.678
Mitomycin-C 
 0.005     
 10 210 243 1.157 24.30 27.0 216.245
Trend: 5.181        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 03/19/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 425 0.405 8.50 26.5 0.000
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
500         
 50 1049 468 0.446 9.36 26.5 10.118 
750         
 50 1049 497 0.474 9.94 26.5 16.941 
1000         
 50 1048 559 0.533 11.18 26.5 31.655 
1250         
 50 1048 605 0.577 12.10 26.5 42.489
Positive Control Mitomycin-C 
 0.001     
 50 1048 603 0.575 12.06 26.5 42.018
Mitomycin-C 
 0.005     
 10 210 248 1.181 24.80 26.5 191.487
Trend: 6.250        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 03/19/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 425 0.405 8.50 26.5 0.000
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
1000         
 50 1042 510 0.489 10.20 26.5 20.806 
1250         
 50 1042 642 0.616 12.84 26.5 52.074 
1500         
 50 1048 727 0.694 14.54 31.5 71.222
Positive Control Mitomycin-C 
 0.001     
 50 1048 603 0.575 12.06 26.5 42.018
Mitomycin-C 
 0.005     
 10 210 248 1.181 24.80 26.5 191.487
Trend: 9.697        
Probability: 0.000        

Trial #: 4    Activation: No Activation    Date: 09/04/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1044 457 0.438 9.14 26.0 0.000
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
750         
 50 1045 551 0.527 11.02 26.0 20.454 
1000         
 50 1053 532 0.505 10.64 26.0 15.416 
1250         
 50 1049 628 0.599 12.56 26.0 36.763 
1500         
 50 1050 758 0.722 15.16 31.0 64.917
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 596 0.568 11.92 26.0 29.671
Mitomycin-C 
 0.005     
 10 211 245 1.161 24.50 26.0 165.258
Trend: 8.499        
Probability: 0.000        

Trial #: 5    Activation: No Activation    Date: 09/04/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1048 405 0.386 8.10 26.0 0.000
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
750         
 50 1048 511 0.488 10.22 26.0 26.173 
1000         
 50 1047 544 0.520 10.88 26.0 34.449 
1250         
 50 1048 691 0.659 13.82 26.0 70.617 
1500         
 50 1049 806 0.768 16.12 31.0 98.823
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 596 0.568 11.92 26.0 46.880
Mitomycin-C 
 0.005     
 10 211 245 1.161 24.50 26.0 200.462
Trend: 12.575        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/07/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1042 404 0.388 8.08 26.0 0.000
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
50         
 50 1041 424 0.407 8.48 26.0 5.051 
160         
 50 1045 415 0.397 8.30 26.0 2.428 
500         
 50 1034 453 0.438 9.06 26.0 12.996 
1600         
 50 1041 496 0.476 9.92 26.0 22.890
Positive Control Cyclophosphamide 
 0.3       
 50 1033 675 0.653 13.50 26.0 68.535
Cyclophosphamide 
 0.6       
 10 207 212 1.024 21.20 26.0 164.151
Trend: 3.272        
Probability: 0.001        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/19/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1042 432 0.415 8.64 26.5 0.000
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
1000         
 50 1045 427 0.409 8.54 26.5 -1.441 
1500         
 50 1047 472 0.451 9.44 26.5 8.737 
2000         
 50 1043 548 0.525 10.96 26.5 26.730 
3000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.2       
 50 1046 711 0.680 14.22 26.5 63.954
Cyclophosphamide 
 0.6       
 10 210 231 1.100 23.10 26.5 165.324
Trend: 3.938        
Probability: 0.000