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Study Information
Study Attribute Value
CEBS Accession Number: 002-02594-0002-0000-4
Chemical Name: Nitrilotriacetic acid trisodium monohydrate
CASRN: 18662-53-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 696583_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 03/28/1985    Harvest Time: 13.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Nitrilotriacetic acid trisodium monohydrate 
1000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
1250         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
1500         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 15 0.150 14.0 7 0.070 6.0 8 0.080 8.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 19 0.380 26.0 15 0.300 20.0 4 0.080 8.0 0 0.000 0.0
Trend: 0.798 0.489 0.627  
Probability: 0.212 0.312 0.265  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 03/28/1985    Harvest Time: 14.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Nitrilotriacetic acid trisodium monohydrate 
1500         
 100 6 0.060 5.0 2 0.020 2.0 4 0.040 4.0 0 0.000 0.0 
2000         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
2500         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
3000         
 100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 100 75 0.750 52.0 51 0.510 37.0 24 0.240 21.0 0 0.000 0.0
Cyclophosphamide 
15         
 50 72 1.440 66.0 43 0.860 54.0 29 0.580 44.0 0 0.000 0.0
Trend: 0.600 0.855 -0.086  
Probability: 0.274 0.196 0.534  


Trial #:2   Activation: No Activation    Date: 03/28/1985    Harvest Time: 13.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Nitrilotriacetic acid trisodium monohydrate 
1000         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1250         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
1500         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2000         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 100 15 0.150 14.0 7 0.070 6.0 8 0.080 8.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 19 0.380 26.0 15 0.300 20.0 4 0.080 8.0 0 0.000 0.0
Trend: 1.111 0.663 1.065  
Probability: 0.133 0.254 0.143  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/28/1985    Harvest Time: 14.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Nitrilotriacetic acid trisodium monohydrate 
1500         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 
2000         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 
2500         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
3000         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
10         
 100 75 0.750 52.0 51 0.510 37.0 24 0.240 21.0 0 0.000 0.0
Cyclophosphamide 
15         
 50 72 1.440 66.0 43 0.860 54.0 29 0.580 44.0 0 0.000 0.0
Trend: 0.376 0.310 0.231  
Probability: 0.353 0.378 0.409