Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02594-0008-0000-0
Chemical Name:	Nitrilotriacetic acid trisodium monohydrate
CASRN:	18662-53-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G05650
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	69	105	38	18	20
			60	108	48	27
			62	93	29	16
			55	94	31	19
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	125	88#	134	30	11	14
			92	103	48	17	14
		250	65	77	41	21	23
			81	87	62	26	23
		500	67	96	38	19	21
			67	81	46	23	21
		750	53	50	25	16	15
			70	52	30	14	15
		1000

Positive Control	Ethyl Methane Sulfonate	500	33	31	498	511	494*
			39	31	560	481
			39	39	570	491

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	91	104	68	25	16
			90	115	29	11
			81	82	27	11
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	250	81	95	81	33	27*
			91	112	57	21	27*
		500	107	87	77	24	19
			101	99	40	13	19
		800	90	57	66	24	19
			94	65	36	13	19
		900	99	38	62	21
			122r	73	88	24
		1000	101	37	79	26	17
			97	40	25	9	17
		1200	84	20	36	14	11
			84	12	19	8	11
Positive Control	Ethyl Methane Sulfonate	500	58	41	862	493	496*
			54	49	867	537
			50	40	690	458

Non-Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90	111	77	28	28
			69#	98	48	23
			83	104	80	32
			85#	88	70	27
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	1000	81	89	59	24	26
			79	99	65	27	26
		1500	80	87	60	25	31
			83	84	91	36	31
		1750	82	101	55	22	22
			120	135	75	21	22
		2000	89	95	60	23	23
			87	94	59	23	23
		2500	80	78	55	23
			121r	70	82	23
		3000	81	45	60	25	23
			98	57	61	21	23
Positive Control	3-Methylcholanthrene	7	61	46	284	155	156*
			81	49	370	152
			65	52	315	161

Non-Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	95	97	65	23	25
			98	102	88	30
			105	104	72	23
			99	96	71	24
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	250	85	81	83	33	28
			92	73	68	25
			97	90	76	26
		500	76	70	78	34	36
			67	69	74	37
			69	81	77	37
		1000	95	91	63	22	23
			78	75	62	27
			109	93	67	21
		2000	122r	38	47	13	20
			84	45	59	23
			97	52	46	16
Positive Control	3-Methylcholanthrene	7	89	55	382	143	135*
			67	50	301	150
			80	60	274	114

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	88	97	90	34	30
			100	114	71	24
			80	86	77	32
			101	103	90	30
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	125	85	110	47	19	21
			81	108	59	24	21
		250	96	118	70	24	24
			93	93	68	24	24
		500	93#	95	79.5	29	30
			81	96	76	31	30
		1000	92	92	72	26	29
			75	74	72	32	29
		1500	76	78	78	34	31
			81	70	70	29	31
		2000	90	30	74	28	29
			83	51	74	30	29
Positive Control	3-Methylcholanthrene	5	44	20	395	302	281*
Positive Control			49	26	382	261	281*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	57	95	124	73	69
			56	94	118	70
			52	72	121	78
			82	139	135	55
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	125	59	113	103	58	62
			58	115	125	71
			61	120	103	56
		250	59	98	80	45	52
			70	110	100	48
			60	118	111	62
		500	50	104	67	45	53
			57	111	101	59
			60	120	99	55
		1000	46	100	85	61	61
			64	85	135	71
			75	84	117	52
		2000	74	60	112	51	48
			56	62	85	51
			48	50	62	44
		3000	107	22	92	29	35
			99	50	120	41

		5000


Positive Control	3-Methylcholanthrene	5	41	17	508	415	426*
			37	11	505	455
			44	22	538	408

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	114	108	136	40	43
			41r	63	80	65
			75	91	97	43
			78	100	111	47
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	250	113	151	65	19	19
			102	141	56	18
			75	118	41	18
		500	114	138	55	16	17
			68	101	28	14
			69	96	43	21
		750	94	120	53	19	19
			63	76	28	15
			96	114	65	23
		1000	98	106	59	20	25
			107	118	103	32
			75	107	53	24
		1500	72	67	46	21	23
			117	114	85	24
			91	139	63	23
		3000	80	90	100	42	32
			89	100	60	22
			68	97	65	32
		5000


Positive Control	3-Methylcholanthrene	5	54	25	368	227	278*
			43	19	432	336
			46	21	369	270

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination