Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02594-0009-0000-1
Chemical Name:	Nitrilotriacetic acid trisodium monohydrate
CASRN:	18662-53-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G89169
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	61	81	109	60	57
			63	104	93	50
			67	95	117	58
			71	121	127	60
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	718	65	87	179	91	82
			71	99	156	73	82
		845	68	85	132	65	60
			69	95	114	55	60
		994	65	40	97	50	58
			66	88	132	67	58
		1170	53	76	105	66	66
			63	78	125	66	66
		1376	64	39	122	64	57
			71	79	107	50	57
		1619	62	29	97	53	55
			67	48	118	58	55
		1900	29	2	71	81	72
			48	8	92	63	72
Positive Control	Ethyl Methane Sulfonate	500	33	36	831	852	532*
			50	42	1025	681
			48	50	92	63

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	73	102	68	31	27
			75	101	75	34
			79	99	52	22
			76	98	51	22
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	524	68#	112	51	25	29
			79	52	80	34	29
		655	89	109	58	22	20
			91	109	57	21
			96	104	53	18
		819	104	141	102	33	37
			71	71	89	42	37
		1024	76	70	72	32	34
			102	107	110	36
			81	94	84	35
		1280	96	101	73	25	31
			74	70	91	41
			105	107	84	27
		1600	80	22	110	46	37
			71	55	75	35
			77	60	70	30
Positive Control	Ethyl Methane Sulfonate	500					613*
			45	44	829	616
			41	34	751	611

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	73	104	62	29	31
			65	86	79	41
			57	91	37	22
			77	119	73	32
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	1043	88	204	78	30	32
			89	177	91	34	32
		1227	81	116	22	9	16
			88	99	61	23	16
		1443	75	78	59	26	23
			89	117	53	20	23
		1698	84	76	43	17	30
			68	53	86	42	30
		1998	63	34	75	40	37
			66	22	69	35	37
		2350	64	14	65	34	35
			48	11	52	36	35
Positive Control	3-Methylcholanthrene	5	56	63	363	217	227*
			54	58	352	217
			52	50	385	247

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	86#	96	160	62	69
			82#	95	171	70
			93	100	196	70
			94	109	204	73
Test Chemical	Nitrilotriacetic acid trisodium monohydrate	630	66	59	206	105	82
			100	91	215	72
			101	93	207	68
		790	97	77	200	69	82
			74	61	210	95	82
		980	79	56	193	81	93
			91	73	183	67
			57	44	224	130
		1230	134r	77	219	54
			79	56	169	72
		1540	87	35	169	65	66
			80	31	176	73
			89	39	158	59
		1920	77	23	188	82	95
			65#	12	195	100
			67	16	208	103
Positive Control	3-Methylcholanthrene	5	79	71	566	240	261*
			72	61	573	267
			72	62	593	276

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination