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Study Information
Study Attribute Value
CEBS Accession Number: 002-02594-0009-0000-1
Chemical Name: Nitrilotriacetic acid trisodium monohydrate
CASRN: 18662-53-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G89169
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 61 81 109 60 57
    63 104 93 50
    67 95 117 58
    71 121 127 60
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
718         
 65 87 179 91 82
    71 99 156 73
  
845         
 68 85 132 65 60
    69 95 114 55
  
994         
 65 40 97 50 58
    66 88 132 67
  
1170         
 53 76 105 66 66
    63 78 125 66
  
1376         
 64 39 122 64 57
    71 79 107 50
  
1619         
 62 29 97 53 55
    67 48 118 58
  
1900         
 29 2 71 81 72
    48 8 92 63
Positive Control Ethyl Methane Sulfonate 
500         
 33 36 831 852 532*
    50 42 1025 681
    48 50 92 63

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 73 102 68 31 27
    75 101 75 34
    79 99 52 22
    76 98 51 22
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
524         
 68# 112 51 25 29
    79 52 80 34
  
655         
 89 109 58 22 20
    91 109 57 21
    96 104 53 18
  
819         
 104 141 102 33 37
    71 71 89 42
  
1024         
 76 70 72 32 34
    102 107 110 36
    81 94 84 35
  
1280         
 96 101 73 25 31
    74 70 91 41
    105 107 84 27
  
1600         
 80 22 110 46 37
    71 55 75 35
    77 60 70 30
Positive Control Ethyl Methane Sulfonate 
500         
 613*
    45 44 829 616
    41 34 751 611

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 73 104 62 29 31
    65 86 79 41
    57 91 37 22
    77 119 73 32
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
1043         
 88 204 78 30 32
    89 177 91 34
  
1227         
 81 116 22 9 16
    88 99 61 23
  
1443         
 75 78 59 26 23
    89 117 53 20
  
1698         
 84 76 43 17 30
    68 53 86 42
  
1998         
 63 34 75 40 37
    66 22 69 35
  
2350         
 64 14 65 34 35
    48 11 52 36
Positive Control 3-Methylcholanthrene 
5         
 56 63 363 217 227*
    54 58 352 217
    52 50 385 247

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 86# 96 160 62 69
    82# 95 171 70
    93 100 196 70
    94 109 204 73
Test Chemical Nitrilotriacetic acid trisodium monohydrate 
630         
 66 59 206 105 82
    100 91 215 72
    101 93 207 68
  
790         
 97 77 200 69 82
    74 61 210 95
  
980         
 79 56 193 81 93
    91 73 183 67
    57 44 224 130
  
1230         
 134r 77 219 54
    79 56 169 72
  
1540         
 87 35 169 65 66
    80 31 176 73
    89 39 158 59
  
1920         
 77 23 188 82 95
    65# 12 195 100
    67 16 208 103
Positive Control 3-Methylcholanthrene 
5         
 79 71 566 240 261*
    72 61 573 267
    72 62 593 276

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination