Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02597-0011-0000-7
Chemical Name:	Nitrofen
CASRN:	1836-75-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	927047
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	98	9	4	7
			76	115	11	5
			62	89	13	7
			74	98	27	12
Test Chemical	Nitrofen	5	61	71	45	25	26*
			61	87	51	28	26*
		10	61	81	50	27	23*
			65	86	38	19	23*
		20	74	70	48	22	25*
			68	78	59	29	25*
		40	64	28	708	368	267*
			76	38	378	166	267*
		80	8r	1	275	1196
			12	2	340	932
Positive Control	Methyl Methane Sulfonate	15	33	28	73	75	58*
			34	30	43	42	58*
	Methyl Methane Sulfonate	250	65	75	233	119	120*
			62	83	224	120	120*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	98	41	16	19
			79	103	43	18
			87	101	50	19
			83	98	56	23
Test Chemical	Nitrofen	20	67	64	79	39	34*
			84	80	73	29	34*
		40	85	56	72	28	36*
			75	54	97	43	36*
		60	19	3	243	434	418*
			21	3	255	402	418*
		80	8r	1	220	880
			6r	1	176	1035
		100	5r	1	160	1103
Positive Control	Methyl Methane Sulfonate	15	33	29	176	179	181*
			35	29	193	183	181*
	Methyl Methane Sulfonate	250	73	72	495	228	210*
			70	64	403	192	210*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	99	160	72	67
			68	84	126	62
			68	113	135	66
			72	104	147	68
Test Chemical	Nitrofen	4	81	21	418	173	177*
			88	19	476	181	177*
		4.5	50	10	306	203	206*
			64	13	401	210	206*
		5	55	7	518	315	269*
			82	10	551	224	269*
		5.5	62	6	700	376	342*
			77	7	710	307	342*
		6	63	5	784	413	497*
			44	4	761	581	497*
Positive Control	Methyl Methane Sulfonate	15	31	17	489	529	553*
Positive Control			28	19	482	577	553*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination