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Study Information
Study Attribute Value
CEBS Accession Number: 002-02597-0011-0000-7
Chemical Name: Nitrofen
CASRN: 1836-75-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 927047
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 98 9 4 7
    76 115 11 5
    62 89 13 7
    74 98 27 12
Test Chemical Nitrofen 
5         
 61 71 45 25 26*
    61 87 51 28
  
10         
 61 81 50 27 23*
    65 86 38 19
  
20         
 74 70 48 22 25*
    68 78 59 29
  
40         
 64 28 708 368 267*
    76 38 378 166
  
80         
 8r 1 275 1196
    12 2 340 932
Positive Control Methyl Methane Sulfonate 
15         
 33 28 73 75 58*
    34 30 43 42
Methyl Methane Sulfonate 
250         
 65 75 233 119 120*
    62 83 224 120

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 98 41 16 19
    79 103 43 18
    87 101 50 19
    83 98 56 23
Test Chemical Nitrofen 
20         
 67 64 79 39 34*
    84 80 73 29
  
40         
 85 56 72 28 36*
    75 54 97 43
  
60         
 19 3 243 434 418*
    21 3 255 402
  
80         
 8r 1 220 880
    6r 1 176 1035
  
100         
 5r 1 160 1103
Positive Control Methyl Methane Sulfonate 
15         
 33 29 176 179 181*
    35 29 193 183
Methyl Methane Sulfonate 
250         
 73 72 495 228 210*
    70 64 403 192

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 74 99 160 72 67
    68 84 126 62
    68 113 135 66
    72 104 147 68
Test Chemical Nitrofen 
4         
 81 21 418 173 177*
    88 19 476 181
  
4.5       
 50 10 306 203 206*
    64 13 401 210
  
5         
 55 7 518 315 269*
    82 10 551 224
  
5.5       
 62 6 700 376 342*
    77 7 710 307
  
6         
 63 5 784 413 497*
    44 4 761 581
Positive Control Methyl Methane Sulfonate 
15         
 31 17 489 529 553*
    28 19 482 577

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination