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Study Information
Study Attribute Value
CEBS Accession Number: 002-02598-0016-0000-3
Chemical Name: Nitrofurantoin
CASRN: 67-20-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 922322
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 126r 108 136 36 35
    129r 120 170 44
    108 84 119 37
    108 116 110 34
Test Chemical Nitrofurantoin 
50         
 99 60 152 51 54
    87 71 149 57
    84 64 134 53
  
100         
 84 39 272 108 101*
    70 44 207 99
    90# 51 257 96
  
150         
 71 26 430 203 196*
    102 25 658 216
    65 29 330 170
  
200         
 75 19 598 268 194*
    66 26 236 120
  
300         
 57 13 516 302 292*
    73 14 669 306
    72 18 575 268
  
500         
 62 10 544 292 298*
    49 8 406 277
    52 9 507 324
Positive Control Methyl Methane Sulfonate 
5         
 81 57 690 285 285*
    89 60 731 275
    71 52 631 296

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 114 80 31 25
    86 97 74 29
    85 85 45 18
    79 104 55 23
Test Chemical Nitrofurantoin 
50         
 87 61 69 26 40*
    93 64 155 56
    74 70 84 38
  
100         
 77 37 184 80 60*
    75 41 155 69
    73 49 73 33
  
150         
 62 26 91 49 78*
    65 28 181 93
    56 29 155 93
  
200         
 50 17 312 207 144*
    48 11 172 121
    81 17 252 104
  
300         
 35 8 239 229 271*
    48 7 369 256
    29 4 283 327
  
500         
 25 2 77 103 374*
    16 2 204 439
    17# 2 287 580
Positive Control Methyl Methane Sulfonate 
5         
 46 38 261 188 149*
    67 50 274 137
    57 44 208 122

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination