Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02599-0012-0000-0
Chemical Name:	Nitrofurazone
CASRN:	59-87-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	267199
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	102	147	54	53
			80	116	149	62
			96	89	128	45
			80	92	120	50
Test Chemical	Nitrofurazone	25	60	51	64	35	34
			55	55	66	40
			69	77	57	27
		50	83#	59	98	39	40
			109	61	110	34
			74	54	106	48
		100	82#	42	145	59	62
			101	38	198	66	62
		150	90	37	254	94	99*
			67#	26	240	119
			86#	39	213	83
		200	83	28	286	115	127*
			75	27	284	127
			74	30	312	140
		300	48	5	517	357	323*
			33	2	357	362
			67#	9	506	250
Positive Control	Methyl Methane Sulfonate	5	61#	50	618	338	294*
			64	57	539	279
			74#	68	589	267

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	91	102	74	27	29
			81	98	68	28
			94	98	74	26
			74	103	73	33
Test Chemical	Nitrofurazone	25	76	71	97	43	37
			75	55	71	32
			73	46	79	36
		50	71	49	104	49	46*
			82	43	102	41
			64	38	94	49
		100	78	39	147	63	65*
			92	40	149	54
			118	36	273	77
		200	78	26	260	111	160*
			76#	9	421	185
			67	8	368	184
		300	48	4	352	247


		400


Positive Control	Methyl Methane Sulfonate	5	74	80	233	106	128*
			93	74	352	126
			77	48	351	153

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination