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Study Information
Study Attribute Value
CEBS Accession Number: 002-02599-0012-0000-0
Chemical Name: Nitrofurazone
CASRN: 59-87-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 267199
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 102 147 54 53
    80 116 149 62
    96 89 128 45
    80 92 120 50
Test Chemical Nitrofurazone 
25         
 60 51 64 35 34
    55 55 66 40
    69 77 57 27
  
50         
 83# 59 98 39 40
    109 61 110 34
    74 54 106 48
  
100         
 82# 42 145 59 62
    101 38 198 66
  
150         
 90 37 254 94 99*
    67# 26 240 119
    86# 39 213 83
  
200         
 83 28 286 115 127*
    75 27 284 127
    74 30 312 140
  
300         
 48 5 517 357 323*
    33 2 357 362
    67# 9 506 250
Positive Control Methyl Methane Sulfonate 
5         
 61# 50 618 338 294*
    64 57 539 279
    74# 68 589 267

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 91 102 74 27 29
    81 98 68 28
    94 98 74 26
    74 103 73 33
Test Chemical Nitrofurazone 
25         
 76 71 97 43 37
    75 55 71 32
    73 46 79 36
  
50         
 71 49 104 49 46*
    82 43 102 41
    64 38 94 49
  
100         
 78 39 147 63 65*
    92 40 149 54
    118 36 273 77
  
200         
 78 26 260 111 160*
    76# 9 421 185
    67 8 368 184
  
300         
 48 4 352 247
   
   
  
400         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 74 80 233 106 128*
    93 74 352 126
    77 48 351 153

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination