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Study Information
Study Attribute Value
CEBS Accession Number: 002-02634-0009-0000-6
Chemical Name: Penicillin V
CASRN: 87-08-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 076958
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 98 92 96 33 31
    93 106 82 29
    99 110 81 27
    88 92 88 34
Test Chemical Penicillin V 
250         
 84 88 70 28 23
    89 91 69 26
    86# 87 37 14
  
500         
 100 82 83 28 23
    106 94 52 16
    116 85 82 24
  
750         
 103 75 87 28 26
    99 67 89 30
    111 71 68 21
  
1000         
 108 69 91 28 27
    105# 77 109 35
    109 71 63 19
  
1500         
 114# 59 66 19 23
    151r 111 104 23
    94 47 77 27
  
2000         
 110 28 160 48 38
    137r 52 101 25
    105 49 86 27
Positive Control Methyl Methane Sulfonate 
5         
 63# 61 481 256 240*
    85 59 518 202
    74# 57 582 262

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 72 94 68 31 28
    104 112 65 21
    82 91 86 35
    110 103 79 24
Test Chemical Penicillin V 
250         
 81 83 58 24 25
    77# 83 59 26
    81 90 62 25
  
500         
 86 107 70 27 27
    94 112 67 24
    92# 87 83 30
  
750         
 94 90 80 28 25
    81 88 55 23
    95 75 66 23
  
1000         
 103 89 97 32 33
    106 101 111 35
    72 101 70 33
  
1500         
 104 72 105 33 30
    98# 103 93 32
    108 84 78 24
  
2000         
 84 83 78 31 35
    108 44 120 37
    85 64 92 36
Positive Control Methyl Methane Sulfonate 
5         
 48 34 426 295 365*
    40 18 428 357
    41 30 549 445

Induced S9 Trial: 1 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 99 107 112 38 44
    66 100 113 57
    113 83 163 48
    100 110 99 33
Test Chemical Penicillin V 
300         
 80 105 88 37 42
    95 104 98 34
    78 108 132 56
  
400         
 115 185 135 39 45
    100 124 151 50
    162r 189 147 30
  
500         
 107 146 134 42 46
    80 60 121 50
    110 156 147 45
  
600         
 97 120 160 55 55
    103 124 126 41
    82 101 167 68
  
1000         
 97 123 154 53 57
    74 113 136 61
    110 118 188 57
Positive Control 3-Methylcholanthrene 
2.5       
   
   

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 118 108 92 26 33
    104 141 101 32
    87# 74 113 43
    86# 77 82 32
Test Chemical Penicillin V 
300         
 77 68 111 48 39
    87 92 103 39
    76 90 66 29
  
400         
 81 85 86 35 31
    88# 91 82 31
    92 104 72 26
  
500         
 84 82 77 30 31
    93 74 94 34
    95 95 83 29
  
600         
 124r 151 81 22 27
    114 104 105 31
    83# 75 60 24
  
800         
 87 93 79 30 31
    76 105 79 35
    80# 72 70 29
  
1000         
 91# 75 99 36 35
    90 85 99 37
    92 90 87 32
Positive Control 3-Methylcholanthrene 
2.5       
 55# 18 716 438 521*
    46 18 676 492
    44# 16 833 633

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 112 102 92 28 27
    91 106 53 20
    93 97 90 32
    85 95 69 27
Test Chemical Penicillin V 
300         
 82# 117 78 32 38
    85 115 103 40
    86 114 108 42
  
400         
 71 108 68 32 25
    113 112 70 21
    102 141 69 23
  
500         
 105# 111 99 31 31
    71 78 81 38
    87 127 64 24
  
600         
 110 73 84 26 32
    110 93 100 30
    92 70 109 39
  
800         
 119 103 75 21 28
    93 85 83 30
    79 53 80 34
  
1000         
 121r 48 72 20 25
    112 77 84 25
    106 78 77 24
Positive Control 3-Methylcholanthrene 
2.5       
 33# 8 305 313 317*
    22# 4 274 412
    45 10 303 225

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination