Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02634-0010-0000-8
Chemical Name:	Penicillin VK
CASRN:	132-98-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	698417
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	70	94	150	72	68
			65	97	138	71
			74	105	158	71
			70	104	125	60
Test Chemical	Penicillin VK	1640	91	114	256	93	93
			58	94	161	92	93
		2050	72	95	233	107	107*
			77	105	248	107	107*
		2560	77	108	270	117	107*
			84	114	247	98	107*
		3200	66	79	259	131	124*
			74	102	258	116	124*
		4000	63	75	248	131	119*
			72	90	233	107	119*
		5000	66	75	226	115	103
			73	84	198	90	103
Positive Control	Ethyl Methane Sulfonate	500	38	34	1025	891	797*
			47	40	1033	733
			41	33	951	767

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	73	121	38	17	26
			73	85	53	24
			68#	108	57	28
			63	86	61	32
Test Chemical	Penicillin VK	2050	79	96	62	26	29
			67	71	63	32	29
		2560	77	116	67	29	36
			55	91	71	43	36
		3200	52	95	45	29	30
			84	134	78	31	30
		4000	65	97	59	30	26
			63	90	41	22	26
		5000	65	93	47	24	30
			64	68	69	36	30
Positive Control	Ethyl Methane Sulfonate	500	41	32	677	550	523*
			46	44	594	432
			38	46	672	587

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	112	98	117	35	44
			123r	108	149	40
			116	112	130	37
			115	90	202	59
Test Chemical	Penicillin VK	2048	143r	121	192	45
			125r	98	163	44
		2560	121r	94	155	43
			98	76	160	54
		3200	105	82	148	47
			122r	85	165	45
		4000	119	95	200	56
			127r	108	195	51
		5000	105	81	175	55	57
			100	80	179	60	57
Positive Control	Ethyl Methane Sulfonate	500	61	42	1104	603	566*
Positive Control			72	46	1143	529	566*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79#	106	249	105	97
			82	108	211	86
			71	84	218	102
			82#	102	231	94
Test Chemical	Penicillin VK	840	75#	60	585	260	243*
			79#	64	536	225	243*
		1050	29#	21	603	701	494*
			77	56	667	288	494*
		1310	65	43	587	302
		1640	28	3	693	835	578*
			72	27	695	320	578*
		2050	51	7	725	471	469*
			54	9	760	466	469*
		3200

Positive Control	3-Methylcholanthrene	5	30	12	656	721	733*
			34	14	744	733
			30#	10	670	744

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	65#	86	112.5	58	57
			66	94	103	52
			74	114	122	55
			78	105	150	64
Test Chemical	Penicillin VK	838	69	74	285	138	145*
			68	67	310	152	145*
		1048	50	47	296	197	183*
			60	56	302	168	183*
		1310	60	50	271	151	166*
			50	38	273	181	166*
		1638	77	36	330	143	162*
			58	31	317	181	162*
		2048	32	6	283	298
Positive Control	3-Methylcholanthrene	5	57	60	465	272	254*
			62	61	411	222
			53	53	428	269

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination