Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02634-0011-0000-9
Chemical Name:	Penicillin VK
CASRN:	132-98-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	954196
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	82	92	59	24	22
			105	104	66	21
			108	117	70	22
			101	87	70	23
Test Chemical	Penicillin VK	500	73	82	59	27	25
			93	84	65	23
			96	70	72	25
		1000	98	74	63	21	25
			91	74	79	29
			85	56	66	26
		2000	88	64	88	33	27
			91	82	53	19
			81	50	66	27
		3000	85	80	61	24	21
			71	65	41	19
			93	75	58	21
		4000	84	70	74	29	27
			74	63	56	25
			85	65	71	28
		5000	103	69	89	29	27
			93	75	71	26
			82#	63	65	27
Positive Control	Ethyl Methane Sulfonate	500	28	6	763	925	990*
			25	7	633	844
			16	5	571	1202

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	104	124	133	43	38
			118	95	121	34
			91	87	102	37
			93	94	111	40
Test Chemical	Penicillin VK	500	89	78	105	39	39
			112	76	129	38
			107	76	122	38
		1000	87	80	100	38	41
			91	72	121	44
			88	79	103	39
		2000	111	76	132	40	40
			79	72	80	34
			112	85	159	47
		3000	95	92	98	35	33
			111	84	95	29
			80	78	83	35
		4000	89	78	132	50	43
			110	84	153	47
			104	90	101	32
		5000	94	81	122	43	39
			79	72	119	51
			105	61	75	24
Positive Control	Ethyl Methane Sulfonate	500	13	2	577	1461	1521*
			11	2	526	1547
			12	2	560	1556

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	103	81	190	61	60
			100	112	130	43
			99	95	215	73
			99	112	180	61
Test Chemical	Penicillin VK	500	86#	49	252	98	79
			98	59	175	60
			94	63	224	80
		1000	83	44	224	90	80
			75	48	183	82
			95	73	193	68
		2000	58#	42	229	131	165*
			70	53	414	199	165*
		3000	63	31	324	171	173*
			80	33	464	195
			88	47	410	155
		4000	79	28	557	234	246*
			68	23	547	270
			70	31	489	235
		5000	57	11	268	156	238*
			20	3	206	346
			82	17	514	210
Positive Control	3-Methylcholanthrene	7	63	19	377	198	229*
			38	12	314	274
			66	15	423	214

Non-Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	84	97	205	82	70
			85	110	154	61
			81	97	155	64
			77	96	167	73
Test Chemical	Penicillin VK	500	98	117	256	87	96
			89	88	263	99
			76	84	233	102
		1000	85	83	230	90	96
			86	80	266	103
			81	63	228	93
		2000	85#	72	297	116	127*
			68	50	295	145
			66	58	234	118
		3000	88	42	458	173	179*
			71	47	405	191
			67	40	350	173
		4000	66	19	376	190	190*
			71	28	414	195
			72	31	394	183
Positive Control	3-Methylcholanthrene	7	64	35	505	264	271*
			75	38	517	229
			54	26	516	319

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	30r	24	189	211	33
			104	126	117	37
			78	109	38	16
			53	65	72	45
Test Chemical	Penicillin VK	78	69	124	50	24	28
			71	125	67	31	28
		156	73	100	55	25	23
			85	130	55	22	23
		313	83	120	82	33	38
			56	85	73	44	38
		625	58	78	103	59	47
			74#	80	78	35	47
		1250	79	72	118	50	44
			85#	74	97.5	38	44
		2500

Positive Control	3-Methylcholanthrene	5	45	11	449	334	427*
			35	9	447	426
			29	7	459	522

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	94	113	105	37	47
			82	84	117	48
			89	92	120	45
			85#	111	151.5	60
Test Chemical	Penicillin VK	125	81	82	119	49	50
			80	82	122	51	50
		250	81	96	133	55	57
			72#	73	126	59	57
		500	97	73	235	81
		1000	79#	80	177	75	95*
			69	68	222	107
			79#	55	245	104
		1500	86	54	253	98	91*
			111#	36	305	92
			89	56	220	82
		2000	85	21	246	97	108*
			74	12	271	122
			69	9	219	106
		3000


Positive Control	3-Methylcholanthrene	5	32	3	367	378	395*
			26	3	324	410
			27	2	321	396

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	98	86	97	33	36
			93	95	87	31
			106	107	99	31
			112	112	158	47
Test Chemical	Penicillin VK	250	88	74	154	58	53*
			79	51	104	44
			85	70	144	56
		500	81	58	143	59	55*
			68	72	88	43
			73	50	139	64
		1000	60	35	111	62	55*
			94	58	129	46
			71	51	123	58
		1500	62	15	259	140	100*
			82	27	178	73
			84	34	218	86
		2000

			61	10	157	86
		2500


Positive Control	3-Methylcholanthrene	5	23	4	189	270	294*
			24	4	189	261
			22	3	232	352

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination