Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02640-0008-0000-2
Chemical Name:	Pentachloroanisole
CASRN:	1825-21-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	644226
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	60	92	48	27	27
			46r	78	58.5	42
			68	108	56	28
Test Chemical	Pentachloroanisole	31.25	81	106	68	28
		62.5	69	92	68	33	28
			80	90	53	22	28
		125	44	27	78	59	50*
			86	76	107	42	50*
		250	118	28	112	32	51*
			102	34	215	70	51*
		500	100	48	120	40	42
			97	32	130	45	42
Positive Control	Ethyl Methane Sulfonate	250	65	78	264	136	145*
Positive Control			57	66	261	154	145*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	63	98	80	42	52
			64	98	94	49
			64	88	99	52
			54	115	107	66
Test Chemical	Pentachloroanisole	31.25	60#	124	66	36	43
			99	127	146	49	43
		62.5	55	97	94	57	52
			72	109	102	47	52
		125	61#	91	115.5	63	63
			69	74	128	62	63
		250	51	25	123	81	71
			87	34	161	62	71
		500	52#	23	133.5	85	83*
			51	18	125	82	83*
Positive Control	Ethyl Methane Sulfonate	250	56	93	413	247	244*
Positive Control			65	79	466	241	244*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	51	94	108	71	71
			68	97	133	66
			79	109	175	74
			63	100	143	76
Test Chemical	Pentachloroanisole	31.25	52	36	523	333	226*
			69	79	247	119	226*
		62.5	64	54	188	99	113*
			84	59	320	126	113*
		125	66	43	149	75	102
			67	105	262	130	102
		250	63	11	220	116	138*
			70#	12	337	160	138*
		500	70	10	191	91	127*
			68	14	332	164	127*
Positive Control	3-Methylcholanthrene	2.5	39	23	739	626	741*
Positive Control			26	21	680	855	741*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92	92	177	64	62
			80	132	105	44
			52	77	122	79
			42r	67	150	118
Test Chemical	Pentachloroanisole	18.75	89	78	302	113	135*
			56	54	264	158	135*
		37.5	58#	53	273	158	137*
			105	82	364	115	137*
		75	109	60	376	115	143*
			56	39	287	171	143*
		150	86	47	340	133	149*
			88	58	436	166	149*
Positive Control	3-Methylcholanthrene	2.5	51	27	956	627	1017*
Positive Control			23#	11	961	1408	1017*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	88	74	39	26
			77	112	45	19
			87	105	57	22
			72	95	50	23
Test Chemical	Pentachloroanisole	50	59	44	144	81	77*
			64	42	141	73	77*
		75	66	29	136	69	55*
			64	34	81	42	55*
		100	57	27	81	47	54*
			55	28	101	61	54*
		125	66	21	114	57	58*
			96	18	171	60	58*
		150	52	20	88	57	57*
			54	16	92	57	57*
Positive Control	3-Methylcholanthrene	2.5	43	24	467	359	371*
Positive Control			44	25	508	383	371*

Induced S9 Trial: 4 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	60	95	57	32	29
			74	90	67	30
			63	103	55	29
			73	111	53	24
Test Chemical	Pentachloroanisole	50	67	42	113	56	49*
			72	41	89	41	49*
		75	62	35	96	52	53*
			71	37	113	53	53*
		100	60	37	56	31	44
			68	37	117	57	44
		125	83	33	89	36	45
			88	36	143	54	45
		150	66	26	80	40	39
			68	32	77	38	39
Positive Control	3-Methylcholanthrene	2.5	69	73	324	158	171*
Positive Control			67	70	374	185	171*

Induced S9 Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	77	104	195	85	78
			65	89	169	87
			57#	81	134	78
			83	127	158	63
Test Chemical	Pentachloroanisole	15	64	79	150	78	72
			79	92	159	67	72
		25	84	65	218	87	84
			76	72	188	82	84
		50	52	40	196	124	145*
			51	32	255	166	145*
		75	37	6	387	350	324*
			36	7	324	299	324*
		100

Positive Control	3-Methylcholanthrene	2.5	46	30	598	436	491*
Positive Control			43	27	700	545	491*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination