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Study Information
Study Attribute Value
CEBS Accession Number: 002-02640-0008-0000-2
Chemical Name: Pentachloroanisole
CASRN: 1825-21-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 644226
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 60 92 48 27 27
    46r 78 58.5 42
    68 108 56 28
Test Chemical Pentachloroanisole 
31.25      
 81 106 68 28
  
62.5       
 69 92 68 33 28
    80 90 53 22
  
125         
 44 27 78 59 50*
    86 76 107 42
  
250         
 118 28 112 32 51*
    102 34 215 70
  
500         
 100 48 120 40 42
    97 32 130 45
Positive Control Ethyl Methane Sulfonate 
250         
 65 78 264 136 145*
    57 66 261 154

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 98 80 42 52
    64 98 94 49
    64 88 99 52
    54 115 107 66
Test Chemical Pentachloroanisole 
31.25      
 60# 124 66 36 43
    99 127 146 49
  
62.5       
 55 97 94 57 52
    72 109 102 47
  
125         
 61# 91 115.5 63 63
    69 74 128 62
  
250         
 51 25 123 81 71
    87 34 161 62
  
500         
 52# 23 133.5 85 83*
    51 18 125 82
Positive Control Ethyl Methane Sulfonate 
250         
 56 93 413 247 244*
    65 79 466 241

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 51 94 108 71 71
    68 97 133 66
    79 109 175 74
    63 100 143 76
Test Chemical Pentachloroanisole 
31.25      
 52 36 523 333 226*
    69 79 247 119
  
62.5       
 64 54 188 99 113*
    84 59 320 126
  
125         
 66 43 149 75 102
    67 105 262 130
  
250         
 63 11 220 116 138*
    70# 12 337 160
  
500         
 70 10 191 91 127*
    68 14 332 164
Positive Control 3-Methylcholanthrene 
2.5       
 39 23 739 626 741*
    26 21 680 855

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 92 177 64 62
    80 132 105 44
    52 77 122 79
    42r 67 150 118
Test Chemical Pentachloroanisole 
18.75      
 89 78 302 113 135*
    56 54 264 158
  
37.5       
 58# 53 273 158 137*
    105 82 364 115
  
75         
 109 60 376 115 143*
    56 39 287 171
  
150         
 86 47 340 133 149*
    88 58 436 166
Positive Control 3-Methylcholanthrene 
2.5       
 51 27 956 627 1017*
    23# 11 961 1408

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64 88 74 39 26
    77 112 45 19
    87 105 57 22
    72 95 50 23
Test Chemical Pentachloroanisole 
50         
 59 44 144 81 77*
    64 42 141 73
  
75         
 66 29 136 69 55*
    64 34 81 42
  
100         
 57 27 81 47 54*
    55 28 101 61
  
125         
 66 21 114 57 58*
    96 18 171 60
  
150         
 52 20 88 57 57*
    54 16 92 57
Positive Control 3-Methylcholanthrene 
2.5       
 43 24 467 359 371*
    44 25 508 383

Induced S9 Trial: 4 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 60 95 57 32 29
    74 90 67 30
    63 103 55 29
    73 111 53 24
Test Chemical Pentachloroanisole 
50         
 67 42 113 56 49*
    72 41 89 41
  
75         
 62 35 96 52 53*
    71 37 113 53
  
100         
 60 37 56 31 44
    68 37 117 57
  
125         
 83 33 89 36 45
    88 36 143 54
  
150         
 66 26 80 40 39
    68 32 77 38
Positive Control 3-Methylcholanthrene 
2.5       
 69 73 324 158 171*
    67 70 374 185

Induced S9 Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 104 195 85 78
    65 89 169 87
    57# 81 134 78
    83 127 158 63
Test Chemical Pentachloroanisole 
15         
 64 79 150 78 72
    79 92 159 67
  
25         
 84 65 218 87 84
    76 72 188 82
  
50         
 52 40 196 124 145*
    51 32 255 166
  
75         
 37 6 387 350 324*
    36 7 324 299
  
100         
   
Positive Control 3-Methylcholanthrene 
2.5       
 46 30 598 436 491*
    43 27 700 545

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination