Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02642-0006-0000-2
Chemical Name:	Pentachloroethane
CASRN:	76-01-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	072127
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	99	124	48	54
			82	101	118	48
			78	113	121	52
			63	87	130	69
Test Chemical	Pentachloroethane	6.25	82	116	109	44	40
			87	118	95	36	40
		12.5	95	103	163	57	48
			82	114	94	38	48
		25	84	110	143	57	48
			78	116	92	39	48
		50	82	89	123	50	55
			71	83	129	61	55
		100	56	50	133	79	66
			71	48	114	53	66
		200

Positive Control	Methyl Methane Sulfonate	15	35	31	173	165	165*
Positive Control			26	25	128	166	165*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	59	124	56	55
			62#	146	120	64
			60#	47	102	57
			57#	149	73.5	43
Test Chemical	Pentachloroethane	70	70#	113	174	83	121*
			42	46	199	158	121*
		90	46	18	190	137
		110	32	13	168	173	126*
			55	78	130	79	126*
		130	63	66	176	94	125*
			41	15	194	157	125*
		150	19#	3	1444	2602	1397*
			55#	13	319	192	1397*
Positive Control	Methyl Methane Sulfonate	15	45#	73	334	246	219*
Positive Control			30	17	170	191	219*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	101	99	210	69	72
			95	89	210	74
			70	104	168	80
			70	108	138	66
Test Chemical	Pentachloroethane	50	79	79	168	71	75
			78	80	183	78	75
		70	83	69	222	89	95
			68	67	207	101	95
		90	96	51	306	106	105
			113	51	352	104	105
		110	79	20	426	181	197*
			87	23	555	213	197*
		130	57	6	1647	960	884*
			56	6	1353	808	884*
		150

Positive Control	Methyl Methane Sulfonate	15	45	28	362	270	262*
Positive Control			48	31	362	254	262*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination