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Study Information
Study Attribute Value
CEBS Accession Number: 002-02643-0012-0000-0
Chemical Name: Pentachloronitrobenzene
CASRN: 82-68-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 303135
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 114 102 115 34 32
    71 95 60 28
    99# 95 101 34
    88 109 86 33
Test Chemical Pentachloronitrobenzene 
1.25      
 94 107 65 23 34
    84# 93 84 33
    71# 73 98 46
  
2.5       
 105 89 67 21 25
    113 84 80 24
    100 81 92 31
  
5         
 102 83 142 46 31
    80 83 53 22
    114 100 83 24
  
10         
 125r 66 127 34 44
    89 63 100 37
    80# 67 121 50
  
20         
 144r 58 154 36 37
    109 58 120 37
    108 55 124 38
  
30         
 100 37 99 33 41
    96# 32 146 51
    87 51 103 40
Positive Control Methyl Methane Sulfonate 
5         
 71 34 616 289 279*
    84 67 637 254
    72 43 632 294

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 117 102 92 26 31
    133r 116 99 25
    112 111 107 32
    100 88 102 34
Test Chemical Pentachloronitrobenzene 
1.25      
 95 88 96 34 30
    86 82 74 29
    94 71 80 28
  
2.5       
 93 84 86 31 28
    103 89 70 23
    89 87 83 31
  
5         
 85 66 88 34 34
    115 80 116 34
    106 81 108 34
  
7.5       
 108 99 63 20 24
    115 101 88 25
    97 89 75 26
  
10         
 112 99 63 19 24
    111 71 85 26
    81 79 69 28
  
15         
 122r 69 111 30 32
    81 65 88 36
    95 77 79 28
Positive Control Methyl Methane Sulfonate 
5         
 89 52 439 164 208*
    57 37 391 230
    72 42 494 229

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 113 117 50 15 23
    91# 84 75 28
    91 102 60 22
    113 97 87 26
Test Chemical Pentachloronitrobenzene 
1.25      
 97 81 80 28 30
    95 64 60 21
    86 65 108 42
  
2.5       
 113 89 88 26 25
    94 81 43 15
    91 79 90 33
  
5         
 104 71 112 36 24
    83 81 46 18
    103 83 57 19
  
7.5       
 94 79 68 24 29
    100 72 79 26
    89 76 97 36
  
10         
 91 59 42 15 17
    102 85 48 16
    111 78 69 21
  
15         
 102# 61 131 43 32
    121r 73 128 35
    117 66 74 21
Positive Control 3-Methylcholanthrene 
2.5       
 52 28 599 380 415*
    66 33 761 383
    45 28 648 480

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 83 104 59 24 27
    102 116 74 24
    75 93 77 34
    86 87 70 27
Test Chemical Pentachloronitrobenzene 
1.25      
 82 81 74 30 28
    86 79 68 26
    82 89 71 29
  
2.5       
 101 90 107 35 33
    79 79 72 30
    81 89 80 33
  
5         
 86# 79 78 30 28
    109 98 85 26
    86 91 71 27
  
7.5       
 109 85 97 30 30
    98 89 89 30
    101 102 87 29
  
10         
 88 92 80 30 31
    99 80 84 28
    90 83 92 34
  
15         
 107 79 80 25 26
    97 86 71 24
    82# 69 72 29
Positive Control 3-Methylcholanthrene 
2.5       
 78 38 778 331 402*
    58 46 625 360
    50 33 775 515

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination