COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02649-0001-0000-4
Chemical Name: Pentamidine isethionate
CASRN: 140-64-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 001715_SCE
Study Result: Weakly Positive

Study Result: Weakly Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 
Induced Rat Liver S9  3_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 02/06/1990    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 408 0.389 8.16 26.0 0.000
Negative (Not Specified) 
0         
 50 1051 370 0.352 7.40 26.0 -9.400
Test Chemical Pentamidine isethionate 
 0.5       
 50 1050 426 0.406 8.52 26.0 4.412 
1.7       
 50 1049 470 0.448 9.40 26.0 15.306 
5         
 50 1051 461 0.439 9.22 26.0 12.883 
17         
 0 0 0 0.000 0.00 31.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1053 596 0.566 11.92 26.0 45.662
Mitomycin-C 
 0.004     
 10 212 186 0.877 18.60 26.0 125.791
Trend: 2.177        
Probability: 0.015        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/06/1990    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 412 0.392 8.24 26.0 0.000
Negative (Not Specified) 
0         
 50 1049 416 0.397 8.32 26.0 1.067
Test Chemical Pentamidine isethionate 
50         
 50 1049 450 0.429 9.00 26.0 9.327 
167         
 50 1048 463 0.442 9.26 26.0 12.593 
500         
 50 1039 493 0.474 9.86 26.0 20.927 
1667         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.125     
 50 1050 576 0.549 11.52 26.0 39.806
Cyclophosphamide 
 0.5       
 10 210 231 1.100 23.10 26.0 180.340
Trend: 2.837        
Probability: 0.002        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 03/15/1990    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 403 0.385 8.06 26.0 0.000
Negative (Not Specified) 
0         
 50 1049 409 0.390 8.18 26.0 1.392
Test Chemical Pentamidine isethionate 
50         
 50 1049 443 0.422 8.86 26.0 9.821 
167         
 50 1050 432 0.411 8.64 26.0 6.992 
500         
 50 1050 569 0.542 11.38 26.0 40.922 
1000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.125     
 50 1047 525 0.501 10.50 26.0 30.397
Cyclophosphamide 
 0.5       
 10 210 281 1.338 28.10 26.0 247.971
Trend: 5.019        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 01/08/1991    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 359 0.343 7.18 26.0 0.000
Negative (Not Specified) 
0         
 50 1050 369 0.351 7.38 26.0 2.590
Test Chemical Pentamidine isethionate 
250         
 50 1047 370 0.353 7.40 26.0 3.163 
500         
 50 1049 414 0.395 8.28 26.0 15.210 
750         
 50 1048 445 0.425 8.90 26.0 23.955 
1000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.125     
 50 1047 530 0.506 10.60 26.0 47.773
Cyclophosphamide 
 0.5       
 10 210 208 0.990 20.80 26.0 189.142
Trend: 3.399        
Probability: 0.000