Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02665-0008-0000-9
Chemical Name:	Phenesterin
CASRN:	3546-10-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	012521
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	102#	119	126	41	33
			86	123	66	25
			81#	29	48	20
			97	130	126	43
Test Chemical	Phenesterin	5	92#	119	108	39	43
			96	133	135	47	43
		10	104	133	111	36
		20	119	123	118	33
			122r	126	112	31
		40	103	129	108	35	34
			107	132	105	33	34
		80	98	135	128	44	37
			99	137	88	30	37
Positive Control	Methyl Methane Sulfonate	15	42	31	281	223	222*
Positive Control			52#	33	345	220	222*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	71	94	40	19	26
			75	93	76	34
			83	101	77	31
			91	112	53	19
Test Chemical	Phenesterin	5	88	108	50	19	23
			81	107	67	28	23
		10	54	94	44	27	24
			78	131	49	21	24
		20	83	122	48	19	27
			63	83	67	35	27
		40	74	97	36	16	22
			104	115	88	28	22
		80	89	97	81	30	22
			86	117	34	13	22
Positive Control	Methyl Methane Sulfonate	15	47#	36	174	123	124*
Positive Control			34#	30	127	125	124*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	91	92	174	64	53
			88	94	133	50
			92	109	132	48
			90	105	138	51
Test Chemical	Phenesterin	10	75	80	155	69	58
			83	91	120	48	58
		20	90	98	167	62	57
			83	87	131	53	57
		40	89	96	142	53	53
			100	110	161	53	53
		60	93	92	125	45	43
			85	80	103	41	43
		80	98	93	112	38	41
			92	105	121	44	41
Positive Control	3-Methylcholanthrene	2.5	37	14	417	377	359*
Positive Control			38	18	385	341	359*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	55	97	134	81	71
			66	113	145	73
			70	98	139	67
			60	92	113	63
Test Chemical	Phenesterin	5	77	92	116	50	50
			69	117	104	50	50
		10	79	100	172	73	72
			73	103	155	70	72
		20	74	98	148	67	71
			77	99	172	75	71
		40	81	94	170	70	69
			78	90	160	68	69
		80	72	95	160	75	76
			57	105	133	78	76
Positive Control	3-Methylcholanthrene	2.5	35	10	581	559	485*
Positive Control			42	11	517	412	485*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination