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Study Information
Study Attribute Value
CEBS Accession Number: 002-02665-0008-0000-9
Chemical Name: Phenesterin
CASRN: 3546-10-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 012521
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 102# 119 126 41 33
    86 123 66 25
    81# 29 48 20
    97 130 126 43
Test Chemical Phenesterin 
5         
 92# 119 108 39 43
    96 133 135 47
  
10         
 104 133 111 36
  
20         
 119 123 118 33
    122r 126 112 31
  
40         
 103 129 108 35 34
    107 132 105 33
  
80         
 98 135 128 44 37
    99 137 88 30
Positive Control Methyl Methane Sulfonate 
15         
 42 31 281 223 222*
    52# 33 345 220

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 71 94 40 19 26
    75 93 76 34
    83 101 77 31
    91 112 53 19
Test Chemical Phenesterin 
5         
 88 108 50 19 23
    81 107 67 28
  
10         
 54 94 44 27 24
    78 131 49 21
  
20         
 83 122 48 19 27
    63 83 67 35
  
40         
 74 97 36 16 22
    104 115 88 28
  
80         
 89 97 81 30 22
    86 117 34 13
Positive Control Methyl Methane Sulfonate 
15         
 47# 36 174 123 124*
    34# 30 127 125

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 91 92 174 64 53
    88 94 133 50
    92 109 132 48
    90 105 138 51
Test Chemical Phenesterin 
10         
 75 80 155 69 58
    83 91 120 48
  
20         
 90 98 167 62 57
    83 87 131 53
  
40         
 89 96 142 53 53
    100 110 161 53
  
60         
 93 92 125 45 43
    85 80 103 41
  
80         
 98 93 112 38 41
    92 105 121 44
Positive Control 3-Methylcholanthrene 
2.5       
 37 14 417 377 359*
    38 18 385 341

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 55 97 134 81 71
    66 113 145 73
    70 98 139 67
    60 92 113 63
Test Chemical Phenesterin 
5         
 77 92 116 50 50
    69 117 104 50
  
10         
 79 100 172 73 72
    73 103 155 70
  
20         
 74 98 148 67 71
    77 99 172 75
  
40         
 81 94 170 70 69
    78 90 160 68
  
80         
 72 95 160 75 76
    57 105 133 78
Positive Control 3-Methylcholanthrene 
2.5       
 35 10 581 559 485*
    42 11 517 412

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination