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Study Information
Study Attribute Value
CEBS Accession Number: 002-02667-0001-0000-4
Chemical Name: Phenformin hydrochloride
CASRN: 834-28-6
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 208878_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Questionable 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 10/01/1985    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 429 0.409 8.58 26.0 0.000
Test Chemical Phenformin hydrochloride 
 0.5       
 50 1050 468 0.446 9.36 26.0 8.987 
1.6       
 50 1047 431 0.412 8.62 26.0 0.658 
5         
 50 1047 457 0.436 9.14 26.0 6.730 
16         
 19 398 206 0.518 10.84 26.0 26.562 
50         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1052 521 0.495 10.42 26.0 21.099
Mitomycin-C 
 0.005     
 10 209 248 1.187 24.80 26.0 190.151
Trend: 1.907        
Probability: 0.028        

Trial #: 2    Activation: No Activation    Date: 12/12/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1043 372 0.357 7.44 26.5 0.000
Test Chemical Phenformin hydrochloride 
5         
 50 1049 372 0.355 7.44 26.5 -0.572 
10         
 50 1045 437 0.418 8.74 26.5 17.248 
16         
 50 1049 444 0.423 8.88 26.5 18.672 
22         
 0 0 0 0.000 0.00 0.0 N/A 
22         
 50 1051 420 0.400 8.40 31.5 12.044
Positive Control Mitomycin-C 
 0.0E-4    
 50 1048 629 0.600 12.58 26.5 68.279
Mitomycin-C 
 0.005     
 10 210 289 1.376 28.90 26.5 285.851
Trend: 2.785        
Probability: 0.003        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/01/1985    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 447 0.426 8.94 26.0 0.000
Test Chemical Phenformin hydrochloride 
50         
 50 1049 472 0.450 9.44 26.0 5.593 
160         
 50 1050 448 0.427 8.96 26.0 0.128 
500         
 50 1049 505 0.481 10.10 26.0 12.975 
1600         
 50 1048 497 0.474 9.94 26.0 11.292
Positive Control Cyclophosphamide 
 0.15      
 50 1049 728 0.694 14.56 26.0 62.864
Cyclophosphamide 
 0.6       
 10 210 280 1.333 28.00 26.0 212.901
Trend: 1.941        
Probability: 0.026