Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02670-0011-0000-9
Chemical Name:	Phenol
CASRN:	108-95-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	987728
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	57	44	152	89	92
			70	87	210	100
			57	117	170	99
			74	152	179	81
Test Chemical	Phenol	100	71	128	204	95	92
			84	138	222	88	92
		200	79	116	192	81	77
			79	139	174	74	77
		400	69	90	251	121	104
			89	112	234	87	104
		800	66	48	267	134	128
			81	48	297	122	128
		1600	77	39	195	85	91
			75	34	219	98	91
		3200

Positive Control	Methyl Methane Sulfonate	15	34	30	289	281	262*
Positive Control			36	25	265	243	262*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	112	68	26	27
			77	97	61	26
			60	86	64	36
			88	106	52	20
Test Chemical	Phenol	600	70	40	96	45	49*
			64	37	100	52	49*
		900	60	22	88	49	49*
			57	23	83	48	49*
		1200	58	19	70	40	37
			60	23	62	35	37
		1500	67	19	110	55	49*
			55	7	71	43	49*
		1800	60	8	92	51	49
			59	7	81	46	49
		2100

Positive Control	Methyl Methane Sulfonate	15	38	28	141	125	115*
Positive Control			47	37	147	104	115*

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	93	53	25	28
			74	86	58	26
			75	105	63	28
			79	117	77	33
Test Chemical	Phenol	300	50	12	97	65	81*
			46#	16	135	97	81*
		600	49	16	92	63	58*
			58	11	93	54	58*
		900	57	11	73	43	45*
			75	14	103	46	45*
		1200	38	9	53	46	43
			45	10	53	39	43
		1500	51	6	101	66	57*
			44	5	64	49	57*
		1800

Positive Control	3-Methylcholanthrene	2.5	40	18	430	361	336*
Positive Control			37	22	347	310	336*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	95	151	53	51
			75	105	117	52
			94	103	140	50
			84	97	120	47
Test Chemical	Phenol	600	80	19	366	153	147*
			61	16	254	140	147*
		900	79	13	404	171	177*
			69	18	382	184	177*
		1200	86	12	425	164	169*
			62	17	321	174	169*
		1500

Positive Control	3-Methylcholanthrene	2.5	73	33	469	214	220*
Positive Control			57	33	386	226	220*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination