Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02679-0009-0000-5
Chemical Name:	Phenylbutazone
CASRN:	50-33-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	421302
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	110	100	78	24	36
			116	98	131	38
			125r	92	156	42
			111	102	152	46
Test Chemical	Phenylbutazone	200	89	170	96	36	29
			108	76	82	25
			116	104	87	25
		300	89	78	93	35	36
			89	80	100	37
			95	75	106	37
		400	96	65	89	31	33
			96	70	85	30
			87	69	103	40
		500	111	38	189	57	85*
			98	28	270	92
			75	21	242	108
		600	72	8	434	200	155*
			79	8	522	221
			104	60	134	43
		800


Positive Control	Methyl Methane Sulfonate	5	89	44	768	287	235*
			93	45	628	225
			85	50	488	191

Nonactivation Trial: 2 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	98	83	143	49	32
			108	97	75	23
			107	111	106	33
			93	109	63	23
Test Chemical	Phenylbutazone	200	101	87	90	30	30
			90	102	54	20
			88	89	104	39
		300	80	70	92	38	32
			107	76	80	25
			102	93	99	32
		400	113	74	104	31	37
			84	73	96	38
			106	66	136	43
		500	99	65	120	40	38
			104	73	104	33
			90	42	106	39
		600	113	103	117	35	47
			81	68	99	41
			90	52	179	67
Positive Control	Methyl Methane Sulfonate	5	72	53	439	203	243*
			72	55	586	271
			64	43	486	255

Induced S9 Trial: 1 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	70	81	74	35	38
			74	96	86	39
			75	118	97	43
			83	106	91	37
Test Chemical	Phenylbutazone	200	71	94	87	41	38
			82	109	83	34
			73	89	86	39
		300	79	121	98	41	47
			75	95	106	47
			111	91	175	52
		400	89	105	150	56	98*
			81	63	241	100
			91	48	376	138
		500					319*
			106	42	546	172
			48	6	675	466
		600


Positive Control	3-Methylcholanthrene	2.5	92#	98	472	170	202*
			87	57	568	218
			103	41	671	218

Induced S9 Trial: 2 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	113	102	91	27	30
			107	115	76	24
			99	90	94	32
			90	93	99	37
Test Chemical	Phenylbutazone	100	71	59	84	40	35
			97	70	81	28
			86	57	94	36
		200	97	69	74	25	38
			84	72	128	51
			82	79	91	37
		300	88	82	81	31	37
			80	85	98	41
			104	76	125	40
		400	116	79	76	22	33
			85	52	101	40
			103	70	118	38
		500	82	17	352	143	105*
			77	35	275	119
			97	51	156	54
Positive Control	3-Methylcholanthrene	2.5	83	54	605	242	296*
			59	23	595	334
			62	21	577	310

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination