Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02680-0009-0000-7
Chemical Name:	Phenylephrine hydrochloride
CASRN:	61-76-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	106595
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	115	113	118	34	28
			118	96	103	29
			128r	83	118	31
			104	91	62	20
Test Chemical	Phenylephrine hydrochloride	250	82	64	53	22
			123r	73	59	16
			133r	89	66	17
		500	88	46	56	21	17
			116	46	57	16
			90	49	36	13
		750	90	39	73	27	27
			117	37	75	21
			117	46	110	31
		1000	101	26	66	22	27
			109	30	110	34
			107	28	79	25
		1500	76	6	141	62	57*
			81	5	109	45
			76	7	145	64
		1800


Positive Control	Methyl Methane Sulfonate	5	75	38	537	240	233*
			66	34	484	246
			89	48	573	214

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	91	97	88	32	33
			73	90	101	46
			94	95	75	27
			101#	119	84	28
Test Chemical	Phenylephrine hydrochloride	125	85	86	88	35	35
			83	92	74	30
			83	90	99	40
		250	77	83	99	43	42
			81	80	103	42
			69	68	85	41
		500	103	56	72	23	39
			50	50	64	43
			62	45	94	50
		750	81	45	108	45	35
			97	43	92	32
			93	46	83	30
		1000	87	27	125	48	54*
			62	20	121	65
			84	33	120	48
		1500	71	12	149	70	78*
			65	11	162	83
			62	13	151	81
Positive Control	Methyl Methane Sulfonate	5	65	33	635	324	298*
			72	42	627	290
			63	39	527	281

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination