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Study Information
Study Attribute Value
CEBS Accession Number: 002-02688-0008-0000-4
Chemical Name: Phthalamide
CASRN: 88-96-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 472127
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 101 197 75 76
    73 97 170 77
    74 94 171 77
    100 108 218 73
Test Chemical Phthalamide 
3.125     
 58 65 125 72 68
    79 95 150 64
  
6.25      
 73 78 126 58 59
    78 90 140 60
  
12.5       
 72 68 142 65 61
    90 96 155 57
  
25         
 82 72 118 48 55
    84 66 158 63
  
50         
 67 77 127 63 58
    79 84 123 52
Positive Control Methyl Methane Sulfonate 
15         
 60 36 334 187 174*
    53 32 257 162

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 109 89 88 27 30
    90 100 107 39
    74 102 60 27
    77 109 64 28
Test Chemical Phthalamide 
10         
 89 110 78 29 33
    94 105 102 36
  
20         
 84 101 54 21 29
    87 96 94 36
  
30         
 93 105 76 27 30
    87 107 85 33
  
40         
 74 102 55 25 29
    81 98 81 33
  
50         
 80 101 53 22 27
    95 101 88 31
Positive Control Methyl Methane Sulfonate 
15         
 58 31 182 105 110*
    40 25 137 116

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 100 48 19 20
    76 103 35 15
    81 103 52 21
    92 94 69 25
Test Chemical Phthalamide 
10         
 40 58 57 48 35*
    54 75 36 22
  
20         
 46 57 56 41 47*
    37 55 58 52
  
30         
 50 69 52 35 37*
    53 70 62 39
  
40         
 81 106 62 26 29
    50 71 49 32
  
50         
 92 113 58 21 23
    109 119 82 25
Positive Control 3-Methylcholanthrene 
2.5       
 26 16 376 488 392*
    39 26 343 296

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 87 104 41 43
    77 96 121 52
    75 109 115 51
    73 108 63 29
Test Chemical Phthalamide 
10         
 121r 100 126 35
    94 115 90 32
  
20         
 87 83 112 43 40
    108 106 118 36
  
30         
 108 122 84 26 35
    104 103 135 43
  
40         
 84 89 99 39 38
    96 117 109 38
  
50         
 90 105 130 48 38
    96 98 83 29
Positive Control 3-Methylcholanthrene 
2.5       
 52 33 504 322 327*
    60 27 594 333

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination