Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-02690-0009-0000-8 |
Chemical Name: | Phthalic anhydride |
CASRN: | 85-44-9 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 358037 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 68 | 102 | 40 | 20 | 28 |
82 | 105 | 61 | 25 | ||||
64 | 97 | 64 | 33 | ||||
68 | 95 | 72 | 35 | ||||
Test Chemical | Phthalic anhydride | 12.5 | 69 | 89 | 58 | 28 | 26 |
64 | 92 | 47 | 24 | ||||
25 | 71 | 89 | 62 | 29 | 32 | ||
77 | 85 | 80 | 34 | ||||
50 | 50 | 65 | 61 | 41 | 44 | ||
57 | 69 | 80 | 47 | ||||
100 | 62 | 40 | 93 | 50 | 39 | ||
58 | 51 | 48 | 28 | ||||
200 | 51 | 14 | 122 | 80 | 71* | ||
55 | 12 | 104 | 63 | ||||
Positive Control | Methyl Methane Sulfonate | 20 | 9r | 3 | 107 | 420 | |
7r | 3 | 101 | 470 | ||||
Methyl Methane Sulfonate | 250 | 51 | 61 | 427 | 279 | 286* | |
46 | 42 | 404 | 294 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 62 | 93 | 78 | 42 | 54 |
51 | 89 | 118 | 77 | ||||
70 | 115 | 109 | 52 | ||||
60 | 103 | 84 | 47 | ||||
Test Chemical | Phthalic anhydride | 25 | 76 | 115 | 50 | 22 | 41 |
73 | 134 | 131 | 60 | ||||
50 | 75 | 93 | 116 | 51 | 58 | ||
75 | 92 | 144 | 64 | ||||
100 | 59 | 73 | 99 | 56 | 60 | ||
64 | 61 | 123 | 64 | ||||
150 | 66 | 30 | 168 | 85 | 78 | ||
51 | 24 | 109 | 72 | ||||
200 | 40 | 6 | 219 | 183 | 218* | ||
32 | 5 | 239 | 253 | ||||
250 | |||||||
Positive Control | Methyl Methane Sulfonate | 15 | 16 | 14 | 329 | 693 | 684* |
18 | 17 | 365 | 676 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination