Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02702-0008-0000-1
Chemical Name:	Piperonyl butoxide
CASRN:	51-03-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	374054
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97	102	68	23	23
			93	96	71	26
			92	106	43	16
			101	96	81	27
Test Chemical	Piperonyl butoxide	6.25	85	83	60	23	22
			88	78	56	21	22
		12.5	85	65	87	34	29
			102	94	74	24	29
		25	98	62	67	23	25
			107	67	87	27	25
		50	80	18	1172	490	466*
			73	16	962	442	466*
		100

Positive Control	Methyl Methane Sulfonate	15	43	27	119	92	84*
			45	27	103	76	84*
	Methyl Methane Sulfonate	250	121r	106	346	95	112*
			102	106	342	112	112*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	75	110	33	15	26
			79	97	81	34
			88	89	64	24
			84	105	77	31
Test Chemical	Piperonyl butoxide	30	75	63	95	42	43*
			64	57	83	43	43*
		45	61	45	83	45	47*
			96	57	138	48	47*
		60	52	9	805	518	387*
			65	18	495	256	387*
		75	11	1	1173	3555	3288*
			14	1	1254	3022	3288*
		90	3r	0	524	5516
			3r	0	478	5975
Positive Control	Methyl Methane Sulfonate	15	35	18	141	133	109*
			43	29	109	84	109*
	Methyl Methane Sulfonate	250	61	66	285	155	147*
			82	64	341	139	147*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination