Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02703-0010-0000-5
Chemical Name:	Piperonyl sulfoxide
CASRN:	120-62-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	096915
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	108	89	196	60	53
			115	98	165	48
			106	114	163	51
			124r	85	256	69
Test Chemical	Piperonyl sulfoxide	0.005	108	92	103	32	47
			102	79	152	50
			104	83	186	60
		0.01	117	83	142	40	46
			101	70	157	52
			152r	114	151	33
		0.02	110	54	222	67	67
			105#	57	228	73
			111	60	198	60
		0.025	133r	61	217	54
			120r	44	243	67
			106	48	209	66
		0.03	96	31	174	61	65
			124r	31	214	58
			108	16	225	70
		0.035	105	13	258	82	77
			121r	10	255	70
			101	11	217	72
		0.04


Positive Control	Ethyl Methane Sulfonate	250	50	30	858	570	525*
			49	26	848	573
			57	34	735	432

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	84	83	39	37
			90	104	104	39
			89	102	105	39
			98	111	97	33
Test Chemical	Piperonyl sulfoxide	2.5	65#	72	110	57	47
			79	76	97	41
			64	70	81	42
		5	65	81	93	48	45
			77	87	80	34
			74	79	117	53
		10	88	97	104	39	38
			83#	79	82	33
			82	84	105	43
		15	86	74	103	40	45
			87	73	125	48
			87	71	122	47
		20	79	58	130	55	55*
			82	62	129	53
			83	59	143	58
		30	66	14	116	59	58*
			65	19	119	61
			77	17	126	55
Positive Control	Ethyl Methane Sulfonate	250	56	51	723	428	404*
			69	61	778	378
			74	55	897	407

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	102	373	156	145
			70#	108	307.5	147
			89	100	373	140
			77	90	315	136
Test Chemical	Piperonyl sulfoxide	0.625	89	106	340	127	121
			73#	89	261	119
			109	87	383	118
		1.25	93#	85	307	110	136
			75	74	304	135
			73#	69	361	164
		2.5	77	76	306	132	136
			89	50	375	140
			86	64	350	136
		5	66	27	433	219	220*
			74#	41	469	211
			55	23	381	232
		10	60	21	484	270	287*
			62#	21	520	281
			63#	17	587	311
		20	61#	7	568	313	312*
			52#	5	481.5	311	312*
		30


Positive Control	3-Methylcholanthrene	2.5	33#	8	919	935	813*
			36	15	719	660
			33	8	822	843

Induced S9 Trial: 2 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	68#	110	214.5	106	103
			85	100	255	100
			74	90	218	98
			76	101	248	109
Test Chemical	Piperonyl sulfoxide	0.625	72#	91	180	83	82
			104	88	253	81
			89	110	222	83
		1.25	76	77	214	94	93
			72#	71	230	106
			91#	87	212	77
		2.5	77	79	196	85	91
			89	52	247	93
			84	66	245	97
		5	63	27	299	158	155*
			71#	41	307	145
			53	23	256	162
		10	61#	17	386	211	197*
			55	20	314	189
			58#	20	333	190
		20	50#	5	331.5	223	203*
			65#	8	358	184	203*
		30


Positive Control	3-Methylcholanthrene	2.5	29#	8	530	620	537*
			29	8	482	545
			34	14	455	446

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	109	170	72	76
			61#	92	175	96
			81	114	189	78
			72	85	125	58
Test Chemical	Piperonyl sulfoxide	0.313	69#	107	240	117	108
			80	90	236	99

		0.625	82	73	271	111	126*
			68#	83	244	120
			74	72	327	148
		1.25	83	99	228	92	121*
			70	78	331	158
			68	77	231	113
		2.5	58#	58	388	225	175*
			62#	73	282	151
			74	79	335	151
		5	60	37	318	178	208*
			58	27	415	237	208*
		10	65	13	162	83	216*
			38#	6	471	410
			63	15	288	154
		20


Positive Control	3-Methylcholanthrene	2.5	46	20	467	340	432*
			43#	18	439	342
			35#	15	649	614

Induced S9 Trial: 4 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	58#	93	122	70	54
			70	88	89	42
			74	111	117	53
			73	108	111	51
Test Chemical	Piperonyl sulfoxide	0.313	65#	107	148.5	77	70

			76	91	143	63
		0.625	77	73	163	71	78
			67#	88	147	73
			71	74	193	91
		1.25	63	75	142	75	77
			68	80	200	99
			81	103	138	57
		2.5	70	79	187	89	107*
			60#	76	166.5	92
			54#	58	229	141
		5	56	36	187	112	134*
			57	28	264	155	134*
		10	62	13	92	50	133*
			36#	6	264	244
			60	15	187	103
		20


Positive Control	3-Methylcholanthrene	2.5	42	19	303	240	315*
			39#	17	286	243
			30#	14	419	462

Induced S9 Trial: 5 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	91	121	117	43	46
			94#	91	134	48
			94	88	132	47
Test Chemical	Piperonyl sulfoxide	0.625	65#	77	83	43	38
			104	92	110	35
			86	95	91	35
		1.25	82	75	96	39	37
			86	84	94	37
			88	86	89	34
		2.5	99	60	145	49	41
			101	61	114	38
			83	63	90	36
		5	76	23	94	41	47
			83	18	134	54
			78	20	109	46
		10


Positive Control	3-Methylcholanthrene	2.5	93	40	510	182	192*
			82	55	483	196
			75	56	448	199

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination