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Study Information
Study Attribute Value
CEBS Accession Number: 002-02706-0007-0000-4
Chemical Name: Polybrominated biphenyl mixture (Firemaster FF-1)
CASRN: 67774-32-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 152414
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 103# 98 130 42 35
    109 98 113 34
    111 110 110 33
    94 94 89 32
Test Chemical Polybrominated biphenyl mixture (Firemaster FF-1) 
1.25      
 105 20 87 28 30
    114 128 106 31
    101 111 97 32
  
2.5       
 118 91 95 27 28
    114 145 94 27
    91 107 83 30
  
5         
 98 50 107 36 33
    101 44 99 33
    107 155 99 31
  
10         
 110 120 112 34 35
    95 165 112 39
    107 129 103 32
  
20         
 87 95 118 45 38
    103 64 113 37
    117 162 109 31
  
30         
 105 92 81 26 27
    85 32 64 25
    105 57 98 31
Positive Control Methyl Methane Sulfonate 
5         
 92 98 657 239 274*
    73 25 635 290
    71# 77 628 293

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 109 96 62 19 28
    97 97 95 33
    87 114 98 38
    93 93 61 22
Test Chemical Polybrominated biphenyl mixture (Firemaster FF-1) 
1.25      
 96 97 85 30 31
    104 99 113 36
    110 97 85 26
  
2.5       
 100 90 75 25 28
    92# 88 73 26
    84 96 86 34
  
5         
 110 82 136 41 41
    100 76 129 43
    82 86 94 38
  
10         
 88 102 96 36 35
    77 81 83 36
    103 96 99 32
  
20         
 102 98 118 39 36
    103 99 104 34
    110 99 116 35
  
30         
 122r 88 145 40 37
    96 73 116 40
    108 91 112 34
Positive Control Methyl Methane Sulfonate 
5         
 75 45 784 349 285*
    87 71 632 242
    69 44 541 263

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 75 77 81 36 49
    65 73 106 55
    82 140 124 51
    67 110 114 56
Test Chemical Polybrominated biphenyl mixture (Firemaster FF-1) 
2.5       
 97 180 193 67 55
    100 177 168 56
    104 212 137 44
  
5         
 115 230 141 41 42
    100 224 138 46
    115 215 132 38
  
10         
 112 257 140 42 50
    99 192 159 54
    102 238 167 55
  
15         
 120 168 118 33 44
    92 217 143 52
    105 160 145 46
  
20         
 79 181 155 65 64
    122r 256 178 49
    102 243 194 63
  
30         
 121r 198 177 49 49
    104 239 140 45
    96 203 153 53
Positive Control 3-Methylcholanthrene 
2.5       
 88 184 441 167 157*
    103 188 501 162
    78# 62 326 140

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 108# 98 146 45 41
    97 105 99 34
    105 97 142 45
    125r 122 186 50
Test Chemical Polybrominated biphenyl mixture (Firemaster FF-1) 
1.25      
 108 110 145 45 43
    114 123 143 42
    128r 111 123 32
  
2.5       
 120r 83 178 49 37
    109 116 119 36
    118 104 134 38
  
5         
 125r 86 185 49 43
    118 127 148 42
    111 127 149 45
  
10         
 115 93 164 47 52
    115 81 205 60
    117 64 167 48
  
20         
 115 71 146 42 51
    122r 73 175 48
    111 68 199 60
  
30         
 74 33 105 47 45
    114# 67 153 45
    117 94 154 44
Positive Control 3-Methylcholanthrene 
2.5       
 67# 33 834 413 386*
    84# 18 1080 429
    81 35 765 315

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 110 102 136 41 50
    91 86 135 50
    80 112 143 60
Test Chemical Polybrominated biphenyl mixture (Firemaster FF-1) 
1.25      
 100 72 130 44 46
    109 81 148 45
    116 93 170 49
  
2.5       
 100 92 171 57 53
    100 78 161 54
    112 103 158 47
  
5         
 121r 96 186 51 54
    120 93 197 55
    104 118 164 52
  
10         
 92 109 145 53 49
    97 96 141 49
    118 97 163 46
  
20         
 116 87 184 53 49
    117 89 152 43
    120 78 180 50
  
30         
 104 87 156 50 56
    114 72 191 56
    97 78 178 61
Positive Control 3-Methylcholanthrene 
2.5       
 93 68 660 238 239*
    88 55 581 220
    110 66 852 258

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination