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Study Information
Study Attribute Value
CEBS Accession Number: 002-02722-0006-0000-1
Chemical Name: Potassium chloride
CASRN: 7447-40-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 012218
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 86 97 53 21 26
    68 82 65 32
    68 91 49 24
    82 130 67 27
Test Chemical Potassium chloride 
156         
 108 128 49 15 18
    104 122 68 22
  
313         
 89 116 60 22 26
    83# 116 72 29
  
625         
 86 119 51 20 18
    100 133 48 16
  
1250         
 103 120 55 18 18
    91 139 49 18
  
2500         
 95 123 59 21 25
    66 95 57 29
  
5000         
 71 50 81 38 28
    93 79 52 19
Positive Control Ethyl Methane Sulfonate 
500         
 29 14 696 786 862*
    27 21 789 962
    21 16 535 836

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 56 87 30 18 20
    80# 120 64.5 27
    55 83 29 18
    69 110 33 16
Test Chemical Potassium chloride 
500         
 45 56 26 19 16
    62 75 28 15
    58 73 24 14
  
1000         
 54 87 24 15 15
    64 100 34 18
    52 98 19 12
  
2000         
 63 85 32 17 16
    75 83 38 17
    81 82 34 14
  
4000         
 72 59 51 24 18
    63 64 25 13
    63 61 32 17
  
5000         
 65 56 31 16 18
    58 40 37 21
    61 53 32 18
Positive Control Ethyl Methane Sulfonate 
500         
 65 47 531 272 289*
    50 21 428 287
    58 51 532 308

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 100 91 164 54 52
    105 109 153 49
Test Chemical Potassium chloride 
4000         
 97 77 123 42 44
    90 70 104 39
    84 60 130 52
  
5000         
 72# 45 146 68 61
    76# 57 158 69
    107 63 146 46
  
7000         
 84# 33 312 124 133*
    86 26 365 141
  
8000         
 79 12 494 209 199*
    85 11 481 189
  
9000         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 55# 20 962 587 521*
    62# 32 941 503
    71 32 1011 475

Nonactivation Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 98 91 102 35 34
    102 109 104 34
Test Chemical Potassium chloride 
4000         
 83 61 79 32 28
    88 70 60 23
    96 77 82 29
  
5000         
 67# 44 86 43 40
    75# 58 106 47
    105 63 93 30
  
7000         
 85 26 208 81 76*
    85# 34 182 72
  
8000         
 78 13 282 121 115*
    83 11 271 109
  
9000         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 49# 18 754 518 472*
    58# 30 787 456
    64 29 847 441

Non-Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 88 103 97 37 44
    89 85 172 64
    101 126 104 34
    109 85 128 39
Test Chemical Potassium chloride 
500         
 110 140 96 29 33
    93 96 90 32
    82 104 92 38
  
1000         
 97 97 121 42 38
    109 105 125 38
    110 117 113 34
  
2000         
 103 84 122 39 46
    92 61 152 55
    99 76 129 44
  
4000         
 74 24 166 74 73*
    91 28 194 71
    86 27 187 72
  
5000         
 84 16 190 75 69*
    94# 17 219 78
    88 18 146 55
Positive Control 3-Methylcholanthrene 
7         
 68 45 406 200 191*
    73# 44 397.5 183

Non-Induced S9 Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 104 97 91 29 26
    124r 97 77 21
    119 96 116 32
    89 107 41 15
Test Chemical Potassium chloride 
500         
 97 88 78 27 28
    81 62 78 32
    97 71 68 23
  
1000         
 75 72 50 22 21
    106# 87 73.5 23
    92# 83 45 16
  
2000         
 109# 74 86 26 29
    96 60 85 29
    84 67 77 30
  
4000         
 85 33 83 32 39
    84 46 115 46
    92 26 105 38
  
5000         
 104 45 93 30 38
    101 28 151 50
    110 24 111 34
Positive Control 3-Methylcholanthrene 
7         
 51 47 291 192 154*
    81 68 305 125
    67 45 290 145

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 74 101 47 21 24
    91 109 39 14
    66 110 61 31
    62 80 55 29
Test Chemical Potassium chloride 
156         
 95 132 56 20 26
    71 104 67 31
  
313         
 93 114 66 24 22
    98 121 58 20
  
625         
 90 107 74 27 27
    83 87 66 27
  
1250         
 96 114 70 24 22
    86 101 53 21
  
2500         
 70 59 83 40 38
    75 87 80 36
  
5000         
 74 33 116 53 43*
    83 39 83 33
Positive Control 3-Methylcholanthrene 
5         
 25 10 280 371 408*
    23 7 352 510
    32 14 330 344

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 95 100 90 31 28
    95 100 75 26
    89 98 75 28
    104 102 76 24
Test Chemical Potassium chloride 
500         
 78 86 77 33 35
    96 90 114 40
    83 119 82 33
  
1000         
 89 80 85 32 25
    86 79 49 19
    77 79 58 25
  
2000         
 81 102 69 28 29
    97 87 91 31
    97 75 80 28
  
4000         
 69 28 67 33 39
    85 28 117 46
    108 37 125 39
  
5000         
 77 25 161 70 54*
    90 43 100 37
    71 36 116 54
Positive Control 3-Methylcholanthrene 
5         
 52 19 572 365 361*
    56 18 584 351
    54 15 593 367

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 133r 111 85 21 13
    118 110 46 13
    115# 102 54 16
    97 88 30 10
Test Chemical Potassium chloride 
2         
 85 65 33 13 14
    87# 72 30 11
    79# 74 42 18
  
4         
 97 53 49 17 17
    96 53 68 24
    87# 59 30 12
  
5         
 101 49 85 28 22*
    96 38 63 22
    103 51 50 16
  
6         
 103# 34 82.5 27 27*
    174r 68 83 16
    107 26 90 28
  
7         
 99# 17 94.5 32 40*
    87 14 109 42
    94 14 131 47
  
8         
   
  
10         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 105 55 472 149 135*
    109 55 442 135
    106# 58 385.5 122

Induced S9 Trial: 4 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 110 88 190 58 63
    101 97 207 69
    110 115 209 63
    131r 114 251 64
Test Chemical Potassium chloride 
2         
 101 65 158 52 69
    82 61 183 75
    87 49 208 80
  
4         
 87 19 326 125 105*
    74 22 149 68
    69 23 253 123
  
5         
 77 23 207 90 88
    99 18 269 90
    85 18 210 83
  
6         
 80 8 243 102 95*
    64# 10 180 93
    82 9 219 89
  
7         
   
    80# 7 180 75
  
8         
   
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 90 52 614 228 221*
    78# 46 534 228
    84 68 522 208

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination