Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02722-0007-0000-2
Chemical Name:	Potassium chloride
CASRN:	7447-40-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	248742
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	62	126	172	92	92
			82	87	208	85
			80	94	236	99
			70	93	188	90
Test Chemical	Potassium chloride	312.5	104	100	272	87	80
			73	105	160	73	80
		625	88	109	208	79	88
			113	100	326	96	88
		1250	98	100	227	78	74
			96	102	203	71	74
		2500	94	97	211	75	81
			81	99	209	86	81
		5000	84	68	262	104	100
			113	66	326	96	100
Positive Control	Methyl Methane Sulfonate	15	29	21	368	418	436*
Positive Control			32	23	432	455	436*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	63	97	65	35	29
			73	99	42	19
			54	98	54	33
			73	106	61	28
Test Chemical	Potassium chloride	2000	57	73	72	42	37
			64	73	61	32	37
		3000	47	72	56	40	46
			45	77	69	52	46
		4000	41	58	101	82	65*
			34	64	48	47	65*
		5000	46	53	91	66	72*
			32	38	75	77	72*
Positive Control	Methyl Methane Sulfonate	15	34	31	236	230	263*
Positive Control			34	31	305	296	263*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	74	89	81	37	42
			84	117	96	38
			55	89	88	54
			77	105	89	39
Test Chemical	Potassium chloride	2000	83	70	98	39	41
			96	64	125	44	41
		3000	75	69	116	52	46
			80	84	97	40	46
		4000	95	82	124	44	47
			75	65	112	50	47
		5000	108	71	184	57	56
			95	66	157	55	56
Positive Control	Other Positive Control	15	61	42	351	192	190*
Positive Control			51	36	285	188	190*

Nonactivation Trial: 4 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	116#	97	104	30	31
			100#	100	115.5	39
			96#	104	84	29
			119#	99	93	26
Test Chemical	Potassium chloride	2000	95	95	114	40	42
			88#	83	117	44	42
		3000	96#	78	117	41	37
			92	67	93	34	37
		4000	104	57	122	39
			128r	48	117	31
		5000	109#	54	112.5	35	43
			95#	51	147	52	43
Positive Control	Methyl Methane Sulfonate	15	72	41	403	187	187*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	77	81	40	33
			64	104	57	30
			80	110	81	34
			101	109	86	28
Test Chemical	Potassium chloride	2000	75	92	83	37	37
			101	96	112	37	37
		3000	77	109	91	39	37
			105	102	108	34	37
		4000	57	70	70	41	40
			57	82	68	40	40
		5000	54	39	86	54	47
			61	40	72	40	47
Positive Control	3-Methylcholanthrene	2.5	62	49	687	368	370*
Positive Control			52	51	585	373	370*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	107#	90	67	21	22
			86	92	65	25
			95	100	55	19
			111#	117	75	22
Test Chemical	Potassium chloride	1000	74	96	53	24	29
			105#	114	106	34	29
		2000	109#	99	76.5	23	24
			93#	87	70	25	24
		3000	73#	75	64.5	29	29
			79#	104	69	29	29
		5000	55#	75	65	40	34
			71	97	61	29	34
Positive Control	3-Methylcholanthrene	2.5	55#	45	450	274	257*
Positive Control			66	54	473	239	257*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination