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Study Information
Study Attribute Value
CEBS Accession Number: 002-02722-0007-0000-2
Chemical Name: Potassium chloride
CASRN: 7447-40-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 248742
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 62 126 172 92 92
    82 87 208 85
    80 94 236 99
    70 93 188 90
Test Chemical Potassium chloride 
312.5       
 104 100 272 87 80
    73 105 160 73
  
625         
 88 109 208 79 88
    113 100 326 96
  
1250         
 98 100 227 78 74
    96 102 203 71
  
2500         
 94 97 211 75 81
    81 99 209 86
  
5000         
 84 68 262 104 100
    113 66 326 96
Positive Control Methyl Methane Sulfonate 
15         
 29 21 368 418 436*
    32 23 432 455

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 63 97 65 35 29
    73 99 42 19
    54 98 54 33
    73 106 61 28
Test Chemical Potassium chloride 
2000         
 57 73 72 42 37
    64 73 61 32
  
3000         
 47 72 56 40 46
    45 77 69 52
  
4000         
 41 58 101 82 65*
    34 64 48 47
  
5000         
 46 53 91 66 72*
    32 38 75 77
Positive Control Methyl Methane Sulfonate 
15         
 34 31 236 230 263*
    34 31 305 296

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 74 89 81 37 42
    84 117 96 38
    55 89 88 54
    77 105 89 39
Test Chemical Potassium chloride 
2000         
 83 70 98 39 41
    96 64 125 44
  
3000         
 75 69 116 52 46
    80 84 97 40
  
4000         
 95 82 124 44 47
    75 65 112 50
  
5000         
 108 71 184 57 56
    95 66 157 55
Positive Control Other Positive Control 
15         
 61 42 351 192 190*
    51 36 285 188

Nonactivation Trial: 4 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 116# 97 104 30 31
    100# 100 115.5 39
    96# 104 84 29
    119# 99 93 26
Test Chemical Potassium chloride 
2000         
 95 95 114 40 42
    88# 83 117 44
  
3000         
 96# 78 117 41 37
    92 67 93 34
  
4000         
 104 57 122 39
    128r 48 117 31
  
5000         
 109# 54 112.5 35 43
    95# 51 147 52
Positive Control Methyl Methane Sulfonate 
15         
 72 41 403 187 187*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 68 77 81 40 33
    64 104 57 30
    80 110 81 34
    101 109 86 28
Test Chemical Potassium chloride 
2000         
 75 92 83 37 37
    101 96 112 37
  
3000         
 77 109 91 39 37
    105 102 108 34
  
4000         
 57 70 70 41 40
    57 82 68 40
  
5000         
 54 39 86 54 47
    61 40 72 40
Positive Control 3-Methylcholanthrene 
2.5       
 62 49 687 368 370*
    52 51 585 373

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 107# 90 67 21 22
    86 92 65 25
    95 100 55 19
    111# 117 75 22
Test Chemical Potassium chloride 
1000         
 74 96 53 24 29
    105# 114 106 34
  
2000         
 109# 99 76.5 23 24
    93# 87 70 25
  
3000         
 73# 75 64.5 29 29
    79# 104 69 29
  
5000         
 55# 75 65 40 34
    71 97 61 29
Positive Control 3-Methylcholanthrene 
2.5       
 55# 45 450 274 257*
    66 54 473 239

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination