Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02722-0008-0000-3
Chemical Name:	Potassium chloride
CASRN:	7447-40-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	563764
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	114	117	115	34	37
			104	90	83	27
			102	97	136	44
			103	96	138	45
Test Chemical	Potassium chloride	1311	90	97	133	49
			125r	141	112	30
		1638	98	102	144	49	48
			96	100	134	47	48
		2048	135r	125	176	44
			92	86	100	36
		2560	104	92	128	41	40
			106	99	126	40	40
		3200	111	91	143	43	42
			105	85	129	41	42
		4000	113	88	84	25	30
			116	90	121	35	30
		5000	113	69	88	26	32
			99	68	115	39	32
Positive Control	Ethyl Methane Sulfonate	500	54	34	720	442	437*
			56	32	770	460
			66	43	805	409

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	83	94	148	60	76
			84	100	215	85
			83	91	213	86
			92	114	199	72
Test Chemical	Potassium chloride	1049	81	92	578	239	198*
			92	106	430	157	198*
		1311	100	119	593	198	189*
			116	138	626	180	189*
		1638	102	121	577	189	191*
			81	101	466	192	191*
		2048	92	97	454	165	187*
			111	132	692	208	187*
		2560	94	98	480	171	182*
			78	113	455	194	182*
		3200	84	82	466	186	186*
			87	121	489	187	186*
		4000	94	82	1259	445	293*
			91	76	383	141	293*
		5000	93	65	397	142	125*
			89	67	288	108	125*
Positive Control	Ethyl Methane Sulfonate	500	53	37	1114	705	832*
			42	30	1169	939
			41	32	1054	853

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	122r	137	161	44	30
			87	87	78	30
			94	98	110	39
			110	115	69	21
Test Chemical	Potassium chloride	1310	96	76	126	44	44
			87#	62	127.5	49
			97	75	113	39
		1638	73	66	117	54	40
			79	70	70	30
			86	70	93	36
		2048	98	86	65	22	30
			87	80	84	32
			80	56	86	36
		2560	88	72	73	28	35
			88	71	134	51
			90	76	75	28
		3200	94	84	92	33	35
			91	75	104	38
			104	84	104	33
		4000	90	58	107	39	34
			86	49	79	31
			96	59	92	32
		5000	76	27	89	39	35
			89#	30	76.5	29
			85	34	94	37
Positive Control	Ethyl Methane Sulfonate	500	54	35	1004	624	553*
			60#	44	984	551
			68	52	981	484

Induced S9 Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	77	92	385	166	158
			80	93	396	166
			79	106	369	156
			88	109	377	143
Test Chemical	Potassium chloride	1049	89	86	379	142	149
			73	79	343	157	149
		1310	77	71	340	148	119
			75	61	202	90	119
		1638	76	53	390	171	171
			86	65	439	170	171
		2048	70	34	376	180	174
			84	34	424	168	174
		2560	58	17	366	210	196
			69	21	376	181	196
		4000

Positive Control	3-Methylcholanthrene	5	16	2	173	357	356*
			14	2	146	356
			10r	2	155	534

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	59	76	62	35	31
			104	97	93	30
			89	114	71	27
			81	112	75	31
Test Chemical	Potassium chloride	1310	89	98	50	19	24
			79	73	59	25
			83	78	74	30
		1638	65	44	46	24	22
			93	67	73	26
			72	58	38	18
		2048	77	50	33	14	21
			88	40	56	21
			78	42	63	27
		2560	58	18	60	35	33
			60	20	67	37
			71	22	58	27
		3200	56#	9	41	24	35
			49	7	45	31
			45	6	65	49
		4000


Positive Control	3-Methylcholanthrene	5	44	35	411	315	327*
			54	39	511	313
			46#	36	487	353

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	95	101	168	59	65
			83	92	156	63
			92	107	183	67
			91	100	197	72
Test Chemical	Potassium chloride	1638	82	82	197	80	69
			84	77	146	58
			96	88	196	68
		2048	69	61	140	67	104*
			84	78	229	91
			84	74	389	155
		2560	84	70	206	82	78
			87	84	194	74	78
		3200	85	56	267	105	102*
			76	63	198	87
			72	51	249	115
		4000	78	58	247	105	115*
			101	59	373	123
			69	41	241	117
		5000	75	36	364	161	161*
			78	31	395	168
			81	28	370	153
Positive Control	3-Methylcholanthrene	5	53	41	1018	642	482*
			66	58	735	369
			66	50	853	433

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79	93	89	37	45
			101	117	127	42
			94	91	122	43
			94	99	157	56
Test Chemical	Potassium chloride	2391	95	53	158	55	55
			106	54	173	54	55
		2657	105	50	194	61	56
			97	48	147	51	56
		2952	80	27	173	72	79*
			82	30	212	86	79*
		3281	87	24	193	74	74*
			90	22	200	74	74*
		3645	90	17	178	66	92*
			76	13	265	117	92*
		4050	73	9	254	117	127*
			57	7	234	138	127*
		4500	48	3	266	185	166*
			59	4	258	146	166*
		5000

Positive Control	3-Methylcholanthrene	5	79	72	598	253	254*
			90	84	626	232
			74	64	609	276

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination