Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02725-0001-0000-9
Chemical Name:	Potassium fluoride
CASRN:	7789-23-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	712642
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	57	98	95	55	71
Vehicle Control			60	102	156	86	71
Test Chemical	Potassium fluoride	200	62	96	125	68	70
			53	83	95	60
			58	70	144	83
		300	62#	124	121.5	65	65
			64	113	129	68
			57#	101	103	61
		400	66#	86	111	56	58
			65	99	107	55
			74	107	138	62
		500	61	70	163	90	82
			85	83	193	76
			79	90	188	79
		600	66	51	172	87	88
			67	59	170	85
			55#	56	150	92
		800	82#	8	240	97	90
			77	6	203	88
			90	9	228	84
Positive Control	Ethyl Methane Sulfonate	250	47	44	892	628	554*
			44	48	752	572
			52	46	720	463

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	100	96	122	41	45
			86	102	117	46
			105	93	134	42
			100	109	150	50
Test Chemical	Potassium fluoride	300	92	75	148	54	56
			89#	64	165	62
			87#	72	139.5	53
		400	95#	52	228	80	84*
			131r	85	233	59
			70#	39	183	88
		500	95#	52	213	75	72*
			98#	46	202	69
			84	30	179	71
		600	98#	21	270	92	90*
			96#	23	252	87	90*
		700


Positive Control	Ethyl Methane Sulfonate	250	66	34	994	502	526*
			59#	33	1093.5	615
			75	40	1037	460

Nonactivation Trial: 3 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	100	109	108	36	32
			100	96	83	28
			86	102	84	33
			105	93	96	30
Test Chemical	Potassium fluoride	300	92	75	93	34	38
			89#	64	118.5	44
			87#	72	94.5	36
		400	70#	39	103	49	45
			95#	52	117	41
			131r	85	144	37
		500	95#	52	135	48	45
			98#	46	122	41
			84	30	117	46
		600	98#	21	162	55	51*
			96#	23	133.5	46	51*
		700


Positive Control	Ethyl Methane Sulfonate	250	75	40	887	393	437*
			66	34	827	418
			59#	33	888	500

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	81	117	127	52	45
			76	84	113	50
			112	110	137	41
			99	89	116	39
Test Chemical	Potassium fluoride	100	95	106	138	48	48
			87	92	132	51
			104	92	140	45
		200	79	73	92	39	40
			95	92	117	41
			86	90	105	41
		400	100	50	124	41	41
			112	69	155	46
			115	65	119	35
		500	85	41	193	76	73*
			96	51	204	71
			74	45	164	74
		600	49#	11	123	83	76*
			74	21	174	79
			68	35	137	67
		700	85	5	307	120	118*
			80#	4	276	115	118*
		800


Positive Control	Ethyl Methane Sulfonate	250	61#	39	805	438	420*
			63	37	854	455
			73	48	801	366

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	98	103	37	31
			120r	106	110	31
			107	107	94	29
			85#	96	67.5	26
Test Chemical	Potassium fluoride	31.3	84	80	100	40	37
			89#	82	70.5	26
			80	90	110	46
		62.5	100	87	79	26	27
			96	99	79	27
			79	84	68	29
		125	96	84	126	44	39
			101	70	139	46
			94	78	76	27
		250	104	85	137	44	46
			83	67	109	44
			107	64	163	51
		500	92	15	218	79	76*
			88	7	209	79
			81	14	170	70
		750

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	93#	91	239	86	71
			124r	148	184	50
			113	109	188	55
			123r	122	173	47
Test Chemical	Potassium fluoride	50	57	59	158	92	72
			111#	96	171	51	72
		100	77#	80	119	51
		200	90#	91	151	56	63
			86	84	180	70	63
		300	110	89	212	65	70
			93	91	225	81
			96#	92	189	66
		400	89#	52	273	103	106
			77#	44	288	125
			73	56	194	89
		500	104	29	413	133	136*
			93	12	413	149
			70	12	266	127
		600


Positive Control	3-Methylcholanthrene	2.5	77#	48	776	337	313*
			64#	53	780	405
			113	99	671	197

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination