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Study Information
Study Attribute Value
CEBS Accession Number: 002-02725-0001-0000-9
Chemical Name: Potassium fluoride
CASRN: 7789-23-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 712642
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 57 98 95 55 71
    60 102 156 86
Test Chemical Potassium fluoride 
200         
 62 96 125 68 70
    53 83 95 60
    58 70 144 83
  
300         
 62# 124 121.5 65 65
    64 113 129 68
    57# 101 103 61
  
400         
 66# 86 111 56 58
    65 99 107 55
    74 107 138 62
  
500         
 61 70 163 90 82
    85 83 193 76
    79 90 188 79
  
600         
 66 51 172 87 88
    67 59 170 85
    55# 56 150 92
  
800         
 82# 8 240 97 90
    77 6 203 88
    90 9 228 84
Positive Control Ethyl Methane Sulfonate 
250         
 47 44 892 628 554*
    44 48 752 572
    52 46 720 463

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 100 96 122 41 45
    86 102 117 46
    105 93 134 42
    100 109 150 50
Test Chemical Potassium fluoride 
300         
 92 75 148 54 56
    89# 64 165 62
    87# 72 139.5 53
  
400         
 95# 52 228 80 84*
    131r 85 233 59
    70# 39 183 88
  
500         
 95# 52 213 75 72*
    98# 46 202 69
    84 30 179 71
  
600         
 98# 21 270 92 90*
    96# 23 252 87
  
700         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 66 34 994 502 526*
    59# 33 1093.5 615
    75 40 1037 460

Nonactivation Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 100 109 108 36 32
    100 96 83 28
    86 102 84 33
    105 93 96 30
Test Chemical Potassium fluoride 
300         
 92 75 93 34 38
    89# 64 118.5 44
    87# 72 94.5 36
  
400         
 70# 39 103 49 45
    95# 52 117 41
    131r 85 144 37
  
500         
 95# 52 135 48 45
    98# 46 122 41
    84 30 117 46
  
600         
 98# 21 162 55 51*
    96# 23 133.5 46
  
700         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 75 40 887 393 437*
    66 34 827 418
    59# 33 888 500

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 81 117 127 52 45
    76 84 113 50
    112 110 137 41
    99 89 116 39
Test Chemical Potassium fluoride 
100         
 95 106 138 48 48
    87 92 132 51
    104 92 140 45
  
200         
 79 73 92 39 40
    95 92 117 41
    86 90 105 41
  
400         
 100 50 124 41 41
    112 69 155 46
    115 65 119 35
  
500         
 85 41 193 76 73*
    96 51 204 71
    74 45 164 74
  
600         
 49# 11 123 83 76*
    74 21 174 79
    68 35 137 67
  
700         
 85 5 307 120 118*
    80# 4 276 115
  
800         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 61# 39 805 438 420*
    63 37 854 455
    73 48 801 366

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 98 103 37 31
    120r 106 110 31
    107 107 94 29
    85# 96 67.5 26
Test Chemical Potassium fluoride 
31.3       
 84 80 100 40 37
    89# 82 70.5 26
    80 90 110 46
  
62.5       
 100 87 79 26 27
    96 99 79 27
    79 84 68 29
  
125         
 96 84 126 44 39
    101 70 139 46
    94 78 76 27
  
250         
 104 85 137 44 46
    83 67 109 44
    107 64 163 51
  
500         
 92 15 218 79 76*
    88 7 209 79
    81 14 170 70
  
750         
   
   

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 93# 91 239 86 71
    124r 148 184 50
    113 109 188 55
    123r 122 173 47
Test Chemical Potassium fluoride 
50         
 57 59 158 92 72
    111# 96 171 51
  
100         
 77# 80 119 51
  
200         
 90# 91 151 56 63
    86 84 180 70
  
300         
 110 89 212 65 70
    93 91 225 81
    96# 92 189 66
  
400         
 89# 52 273 103 106
    77# 44 288 125
    73 56 194 89
  
500         
 104 29 413 133 136*
    93 12 413 149
    70 12 266 127
  
600         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 77# 48 776 337 313*
    64# 53 780 405
    113 99 671 197

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination