Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02734-0008-0000-6
Chemical Name:	Procarbazine hydrochloride
CASRN:	366-70-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G11149
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	70	129	31	15	17
Vehicle Control			71	106	41	19	17
Test Chemical	Procarbazine hydrochloride	313	67	31	27	13	14
			65	39	29	15	14
		625	50	50	41	27	23
			58	30	32	18	23
		1250	44	46	31	23	24
			52	61	37	24	24
		2500	65	43	45	23	20
			55	49	28	17	20
		5000	61	12	69	38	41*
			49	15	65	44	41*
Positive Control	Ethyl Methane Sulfonate	500	20	12	375	625	789*
Positive Control			11	3	324	953	789*
Negative Control	Negative (Not Specified)	0	76	91	32	14	12*
Negative Control			76	75	22	10	12*

Nonactivation Trial: 2 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	100	97	55	18	17
Vehicle Control			94	106	43	15	17
Test Chemical	Procarbazine hydrochloride	625	95	106	54	19	23
			97	106	77	27	23
		1250	96	111	80	28	27
			93	91	75	27	27
		2500	88	75	98	37	35*
			76	78	73	32	35*
		6000	56	5	98	58	58*
			80	15	138	57	58*
Positive Control	Ethyl Methane Sulfonate	5000
Positive Control
Negative Control	Negative (Not Specified)	0	94	91	53	19	17*
Negative Control			93	107	40	14	17*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	96	103	50	17	18
			102	126	39	13
			79	86	63	27
			79	86	32	14
Test Chemical	Procarbazine hydrochloride	500	92	69	48	17	15
			95	88	38	13	15
		1000	72	66	42	19	17
			92	91	43	16	17
		2000	93	74	53	19	23
			92	72	76	28	23
		3000	96	68	53	18	23
			80	64	69	29
			85	70	53	21
		4000	96	58	65	22	18
			75	56	29	13
			75	64	41	18
		5000	68	30	72	35	37*
			82	50	85	35
			52	35	65	42
Positive Control	Ethyl Methane Sulfonate	500	41	31	637	516	455*
			51	35	629	408
			52	40	695	441

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	104#	105	129	41	36
			96	94	108	37
			114	108	112	33
			101	94	93	31
Test Chemical	Procarbazine hydrochloride	250	101#	88	136	45	44
			88	90	111	42
			83	103	111	45
		500	98	101	89	30	33
			81	96	98	40
			103	113	91	29
		1000	100	87	125	42	38
			98	78	107	37
			90	97	93	35
		2000	108	91	133	41	44
			111	82	150	45
			91	93	128	47
		3000	102	77	194	63	60*
			109	71	163	50
			92#	63	183	67
		4000	68#	14	200	98	112*
			57#	11	165	97
			57	4	240	141
		5000


Positive Control	Ethyl Methane Sulfonate	250	70	42	1019	486	506*
			60	40	952	529
			67	37	1008	504

Induced S9 Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	68	74	57	28	23
Vehicle Control			74	78	42	19	23
Test Chemical	Procarbazine hydrochloride	156	61	41	91	50	62*
			52	34	117	75	62*
		313	41	55	78	63	78*
			39	36	109	92	78*
		625	52	103	71	45	60*
			50	78	112	75	60*
		1250	83	70	126	51	58*
			65	69	125	64	58*
		2500	47	38	86	61	78*
			35	28	99	94	78*
		5000

Positive Control	3-Methylcholanthrene	5	37	14	154	138	162*
Positive Control			32	14	178	187	162*
Negative Control	Negative (Not Specified)	0	74	97	33	15	12*
Negative Control			99	151	28	9	12*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	58	115	44	25	28
Vehicle Control			52	62	49	32	28
Test Chemical	Procarbazine hydrochloride	750	57	130	75	44	39
			65	129	65	34	39
		1500	39	100	51	43	46
			46	95	66	48	46
		3000	54	101	74	46	45
			69	102	91	44	45
		4000	41	73	91	74	73*
			40	51	86	72	73*
		5000	47	72	109	77	79*
			39	54	94	81	79*
Positive Control	3-Methylcholanthrene	5	46	25	325	238	238*
Positive Control			47	23	334	238	238*
Negative Control	Negative (Not Specified)	0	65	131	53	27	28*
Negative Control			51	92	44	29	28*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	77	101	263	115	106
			78	95	280	120
			77	104	189	82
Test Chemical	Procarbazine hydrochloride	187.5	19	9	162	287	291*
			15	8	131	294	291*
		375	37	21	283	253	297*
			17	11	178	342	297*
		750	26	19	227	287	277*
			39	32	312	267	277*
		1500	41	18	359	293	289*
			29	19	252	285	289*
		3000

Positive Control	3-Methylcholanthrene	5					372*
			19	3	232	418
			13	2	130	325

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	98	99	63	22	23
			70	88	48	23
			83	109	55	22
			93	104	76	27
Test Chemical	Procarbazine hydrochloride	125	36r	27	0	0
			41	36	120	97
		500	57	67	147	85	85*
			65	58	165	85	85*
		1000	59	62	141	80	73*
			69	84	137	67	73*
		2000	107	113	210	66	78*
			72	88	162	76
			88	76	243	93
		4000	69	66	222	108	99*
			75	62	202	90	99*
		5000	68	56	232	114	106*
			97	53	289	99	106*
Positive Control	3-Methylcholanthrene	5	50	20	409	272	327*
			43	27	365	282
			32	15	407	428

Induced S9 Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	64	103	63	33	25
			58	107	31	18
			62	104	47	25
			61	86	47	26
Test Chemical	Procarbazine hydrochloride	2.5	46	45	95	69	89*
			35	40	114	109	89*
		5	17	17	86	170	181*
			19	17	107	191	181*
		10	15	9	131	291	309*
			15	8	150	326	309*
		25	11	7	143	427	312*
			18	11	107	196	312*
		50	20	13	132	220	206*
			14	13	82	193	206*
		100	28	22	110	133	142*
			23	18	105	152	142*
Positive Control	3-Methylcholanthrene	5	37	23	351	321	315*
			35	25	305	289
			35	21	352	335

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination