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Study Information
Study Attribute Value
CEBS Accession Number: 002-02734-0008-0000-6
Chemical Name: Procarbazine hydrochloride
CASRN: 366-70-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G11149
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 70 129 31 15 17
    71 106 41 19
Test Chemical Procarbazine hydrochloride 
313         
 67 31 27 13 14
    65 39 29 15
  
625         
 50 50 41 27 23
    58 30 32 18
  
1250         
 44 46 31 23 24
    52 61 37 24
  
2500         
 65 43 45 23 20
    55 49 28 17
  
5000         
 61 12 69 38 41*
    49 15 65 44
Positive Control Ethyl Methane Sulfonate 
500         
 20 12 375 625 789*
    11 3 324 953
Negative Control Negative (Not Specified) 
 0         
 76 91 32 14 12*
    76 75 22 10

Nonactivation Trial: 2 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 100 97 55 18 17
    94 106 43 15
Test Chemical Procarbazine hydrochloride 
625         
 95 106 54 19 23
    97 106 77 27
  
1250         
 96 111 80 28 27
    93 91 75 27
  
2500         
 88 75 98 37 35*
    76 78 73 32
  
6000         
 56 5 98 58 58*
    80 15 138 57
Positive Control Ethyl Methane Sulfonate 
5000         
   
Negative Control Negative (Not Specified) 
 0         
 94 91 53 19 17*
    93 107 40 14

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 96 103 50 17 18
    102 126 39 13
    79 86 63 27
    79 86 32 14
Test Chemical Procarbazine hydrochloride 
500         
 92 69 48 17 15
    95 88 38 13
  
1000         
 72 66 42 19 17
    92 91 43 16
  
2000         
 93 74 53 19 23
    92 72 76 28
  
3000         
 96 68 53 18 23
    80 64 69 29
    85 70 53 21
  
4000         
 96 58 65 22 18
    75 56 29 13
    75 64 41 18
  
5000         
 68 30 72 35 37*
    82 50 85 35
    52 35 65 42
Positive Control Ethyl Methane Sulfonate 
500         
 41 31 637 516 455*
    51 35 629 408
    52 40 695 441

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 104# 105 129 41 36
    96 94 108 37
    114 108 112 33
    101 94 93 31
Test Chemical Procarbazine hydrochloride 
250         
 101# 88 136 45 44
    88 90 111 42
    83 103 111 45
  
500         
 98 101 89 30 33
    81 96 98 40
    103 113 91 29
  
1000         
 100 87 125 42 38
    98 78 107 37
    90 97 93 35
  
2000         
 108 91 133 41 44
    111 82 150 45
    91 93 128 47
  
3000         
 102 77 194 63 60*
    109 71 163 50
    92# 63 183 67
  
4000         
 68# 14 200 98 112*
    57# 11 165 97
    57 4 240 141
  
5000         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 70 42 1019 486 506*
    60 40 952 529
    67 37 1008 504

Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 68 74 57 28 23
    74 78 42 19
Test Chemical Procarbazine hydrochloride 
156         
 61 41 91 50 62*
    52 34 117 75
  
313         
 41 55 78 63 78*
    39 36 109 92
  
625         
 52 103 71 45 60*
    50 78 112 75
  
1250         
 83 70 126 51 58*
    65 69 125 64
  
2500         
 47 38 86 61 78*
    35 28 99 94
  
5000         
   
Positive Control 3-Methylcholanthrene 
5         
 37 14 154 138 162*
    32 14 178 187
Negative Control Negative (Not Specified) 
 0         
 74 97 33 15 12*
    99 151 28 9

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 58 115 44 25 28
    52 62 49 32
Test Chemical Procarbazine hydrochloride 
750         
 57 130 75 44 39
    65 129 65 34
  
1500         
 39 100 51 43 46
    46 95 66 48
  
3000         
 54 101 74 46 45
    69 102 91 44
  
4000         
 41 73 91 74 73*
    40 51 86 72
  
5000         
 47 72 109 77 79*
    39 54 94 81
Positive Control 3-Methylcholanthrene 
5         
 46 25 325 238 238*
    47 23 334 238
Negative Control Negative (Not Specified) 
 0         
 65 131 53 27 28*
    51 92 44 29

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 77 101 263 115 106
    78 95 280 120
    77 104 189 82
Test Chemical Procarbazine hydrochloride 
187.5       
 19 9 162 287 291*
    15 8 131 294
  
375         
 37 21 283 253 297*
    17 11 178 342
  
750         
 26 19 227 287 277*
    39 32 312 267
  
1500         
 41 18 359 293 289*
    29 19 252 285
  
3000         
   
Positive Control 3-Methylcholanthrene 
5         
 372*
    19 3 232 418
    13 2 130 325

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 98 99 63 22 23
    70 88 48 23
    83 109 55 22
    93 104 76 27
Test Chemical Procarbazine hydrochloride 
125         
 36r 27 0 0
    41 36 120 97
  
500         
 57 67 147 85 85*
    65 58 165 85
  
1000         
 59 62 141 80 73*
    69 84 137 67
  
2000         
 107 113 210 66 78*
    72 88 162 76
    88 76 243 93
  
4000         
 69 66 222 108 99*
    75 62 202 90
  
5000         
 68 56 232 114 106*
    97 53 289 99
Positive Control 3-Methylcholanthrene 
5         
 50 20 409 272 327*
    43 27 365 282
    32 15 407 428

Induced S9 Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 64 103 63 33 25
    58 107 31 18
    62 104 47 25
    61 86 47 26
Test Chemical Procarbazine hydrochloride 
2.5       
 46 45 95 69 89*
    35 40 114 109
  
5         
 17 17 86 170 181*
    19 17 107 191
  
10         
 15 9 131 291 309*
    15 8 150 326
  
25         
 11 7 143 427 312*
    18 11 107 196
  
50         
 20 13 132 220 206*
    14 13 82 193
  
100         
 28 22 110 133 142*
    23 18 105 152
Positive Control 3-Methylcholanthrene 
5         
 37 23 351 321 315*
    35 25 305 289
    35 21 352 335

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination