Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02734-0009-0000-7
Chemical Name:	Procarbazine hydrochloride
CASRN:	366-70-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G78218
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	96	102	77	27	26
Vehicle Control			95	98	71	25	26
Test Chemical	Procarbazine hydrochloride	1693	89	83	105	39	40
			91	81	111	41	40
		3385	92	72	114	41	39
			92	67	98	36	39
		3906	93	63	144	52	55*
			84	62	145	58	55*
		4507	88	60	182	69	74*
			86	55	200	78	74*
		5200	95#	54	211	74	90*
			76	46	243	106	90*
		6000	57	22	187	110	123*
			55	21	224	135	123*
Positive Control	Ethyl Methane Sulfonate	500	48	38	1008	700	656*
Positive Control			55	38	1003	612	656*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	94	114	74	26	26
Vehicle Control			94	86	73	26	26
Test Chemical	Procarbazine hydrochloride	846	99	109	112	38
		1693	112	118	107	32
		3385	76	61	117	51
		3906	83	65	147	59
		4507	73	51	183	83	81*
			82	54	195	79	81*
		5200	84	42	230	91	101*
			75	37	249	110	101*
		6000	57	8	263	155	143*
			61	14	237	130	143*
Positive Control	Ethyl Methane Sulfonate	500	52	38	799	511	538*
Positive Control			46	36	786	565	538*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	95	115	43	38
Vehicle Control			97	105	97	33	38
Test Chemical	Procarbazine hydrochloride	846	95	89	95	33	35
			79	81	87	37	35
		1693	73	65	104	48	40
			89	87	86	32	40
		3385	93	70	111	40	32
			114	82	82	24	32
		3906	80	56	90	38	50
			84	56	159	63	50
		4507	90	61	112	42	61
			87	52	211	81	61
		5200	65	20	309	158	146*
			67	18	270	135	146*
		6000

Positive Control	Ethyl Methane Sulfonate	500	42	25	767	616	616*

Induced S9 Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	79	90	291	123	120
Vehicle Control			86	110	302	117	120
Test Chemical	Procarbazine hydrochloride	0.82	86	106	286	111	116
			82	99	297	121	116
		2.05	83	83	327	132	135
			77	83	322	139	135
		5.12	55	52	349	211	189
			63	49	314	167	189
		12.8	23	16	214	312	339*
			19	12	208	365	339*
		32	4r	2	96	768
			8r	5	91	379
		80	3r	2	123	1230
			3r	2	112	1318
Positive Control	3-Methylcholanthrene	5	22	8	441	663	607*
Positive Control			26	10	435	551	607*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination