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Study Information
Study Attribute Value
CEBS Accession Number: 002-02734-0009-0000-7
Chemical Name: Procarbazine hydrochloride
CASRN: 366-70-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G78218
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 96 102 77 27 26
    95 98 71 25
Test Chemical Procarbazine hydrochloride 
1693         
 89 83 105 39 40
    91 81 111 41
  
3385         
 92 72 114 41 39
    92 67 98 36
  
3906         
 93 63 144 52 55*
    84 62 145 58
  
4507         
 88 60 182 69 74*
    86 55 200 78
  
5200         
 95# 54 211 74 90*
    76 46 243 106
  
6000         
 57 22 187 110 123*
    55 21 224 135
Positive Control Ethyl Methane Sulfonate 
500         
 48 38 1008 700 656*
    55 38 1003 612

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 114 74 26 26
    94 86 73 26
Test Chemical Procarbazine hydrochloride 
846         
 99 109 112 38
  
1693         
 112 118 107 32
  
3385         
 76 61 117 51
  
3906         
 83 65 147 59
  
4507         
 73 51 183 83 81*
    82 54 195 79
  
5200         
 84 42 230 91 101*
    75 37 249 110
  
6000         
 57 8 263 155 143*
    61 14 237 130
Positive Control Ethyl Methane Sulfonate 
500         
 52 38 799 511 538*
    46 36 786 565

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 95 115 43 38
    97 105 97 33
Test Chemical Procarbazine hydrochloride 
846         
 95 89 95 33 35
    79 81 87 37
  
1693         
 73 65 104 48 40
    89 87 86 32
  
3385         
 93 70 111 40 32
    114 82 82 24
  
3906         
 80 56 90 38 50
    84 56 159 63
  
4507         
 90 61 112 42 61
    87 52 211 81
  
5200         
 65 20 309 158 146*
    67 18 270 135
  
6000         
   
Positive Control Ethyl Methane Sulfonate 
500         
 42 25 767 616 616*

Induced S9 Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 79 90 291 123 120
    86 110 302 117
Test Chemical Procarbazine hydrochloride 
 0.82      
 86 106 286 111 116
    82 99 297 121
  
2.05      
 83 83 327 132 135
    77 83 322 139
  
5.12      
 55 52 349 211 189
    63 49 314 167
  
12.8       
 23 16 214 312 339*
    19 12 208 365
  
32         
 4r 2 96 768
    8r 5 91 379
  
80         
 3r 2 123 1230
    3r 2 112 1318
Positive Control 3-Methylcholanthrene 
5         
 22 8 441 663 607*
    26 10 435 551

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination