Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02736-0002-0000-2
Chemical Name:	Progesterone
CASRN:	57-83-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	303263
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96#	100	213	74	89
			88	102	253	96
			96#	103	255	89
			82	95	240	98
Test Chemical	Progesterone	27	75	54	268	120	123
			70	75	266	127	123
		36	74	58	189	86	97
			63	68	207	109	97
		47	61	62	254	139	105
			101	58	218	72	105
		63	68	42	174	86	77
			82	47	166	68	77
		84
		112.5

Positive Control	Ethyl Methane Sulfonate	500	56#	54	1113	668	677*
			45	37	881	660
			50#	40	1058	702

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	129	211	81	84
			75	123	208	92
			72	91	169	78
			59	56	149	84
Test Chemical	Progesterone	32	70	94	258	123	105
			62	82	201	109
			55	64	137	84
		40	63	48	208	110	109
			79	76	187	79
			62	61	255	138
		50	57	31	165	96	105
			82#	65	265	107
			75	62	251	111
		63	64	25	192	100	137*
			58	19	246	142
			55	17	282	170
		78


Positive Control	Ethyl Methane Sulfonate	500	52	75	457	294	287*
			63	79	462	246
			56	79	544	323

Induced S9 Trial: 1 Experiment Call:
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	89	233	94	92
			97	107	275	95
			96	104	249	87
Test Chemical	Progesterone	36	88	120	219	83	94
			81	103	253	104	94
		47	85	130	188	74	82
			82	100	221	89	82
		63	86	102	262	102	94
			83	113	212	86	94
		84	71	77	193	90	80
			84	112	175	69	80
		112.5	86	91	206	80	79
			71	80	163	77	79
		150	70	7	229	110	94
			68	12	158	77	94
		200

Positive Control	3-Methylcholanthrene	5	69	66	511	247	250*
			82	79	611	248
			72	74	546	253

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	113	108	321	95	87
			95	92	222	78
			125r	120	297	80
Test Chemical	Progesterone	50	64	81	204	107	108
			73	79	204	93
			71	70	264	124
		63	91	90	226	83	93
			80	86	245	103
			135r	150	272	67
		78	88	92	263	100	92
			109	110	265	81
			89	111	252	95
		98	71	68	211	99	97
			92	81	319	115
			99	96	231	78
		122	83	40	238	96	108
			96	32	300	104
			81	25	298	122
		175


Positive Control	3-Methylcholanthrene	5	94	75	638	225	235*
Positive Control			74	56	541	244	235*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination