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Study Information
Study Attribute Value
CEBS Accession Number: 002-02736-0003-0000-3
Chemical Name: Progesterone
CASRN: 57-83-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 820917
Study Result: Equivocal


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 97 76 29 19
    78 98 42 18
    78 105 23 10
Test Chemical Progesterone 
10         
 77 86 38 17 19
    82 79 45 18
    73 83 47 21
  
20         
 49# 52 37.5 25 21
    83 71 50 20
    76 82 38 17
  
30         
 72# 51 44 20 20
    69 50 44 21
    66 49 35 18
  
40         
 71 17 44 21 22
    68 21 48 24
  
50         
   
   
   
Positive Control Ethyl Methane Sulfonate 
500         
 32 17 865 901 1018*
    20 13 675 1134

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 115 52 22 19
    79# 114 58.5 25
    65 81 26 13
    63 90 33 17
Test Chemical Progesterone 
10         
 57 70 28 16 19
    79 104 29 12
    59 83 51 29
  
20         
 58 79 32 18 26
    62 61 42 23
    62 63 67 36
  
30         
 67 65 44 22 25
    58 22 57 33
    72 39 43 20
  
40         
  
50         
   
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 37 38 494 445 533*
    28# 26 528 621

Nonactivation Trial: 3 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 72 84 83 38 30
    103 116 70 23
Test Chemical Progesterone 
20         
 73 84 99 45 34
    95 90 65 23
  
30         
 93 97 65 23 25
    83 69 56 22
    68# 72 61.5 30
  
40         
 97 55 88 30 29
    91# 51 61 22
    114# 44 98 29
    99 85 104 35
  
50         
 78# 25 131 56 52*
    69 12 94 45
    115 23 164 48
    75 15 133 59
Positive Control Ethyl Methane Sulfonate 
250         
 92 53 1115 404 375*
    81# 43 916.5 380
    94 46 961 341

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 112 166 56 49
    89# 92 118 44
    85# 86 136 53
    92 110 116 42
Test Chemical Progesterone 
10         
 77 78 79 34 36
    87 74 95 37
    78 81 88 38
  
20         
 98 86 89 30 31
    103 75 92 30
    84 86 83 33
  
30         
 79# 63 134 56 49
    88 60 107 41
  
40         
 77 11 290 126 103*
    78 13 258 111
    79 15 199 85
    69 13 192 93
  
50         
   
   
    34 2 237 231
  
60         
   
   
Positive Control Ethyl Methane Sulfonate 
250         
 46# 30 706 517 462*
    55 43 673 409
    44 42 610 460

Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 113 116 154 46 46
    107 99 141 44
    97 93 122 42
    89 92 140 52
Test Chemical Progesterone 
1.56      
 82 77 106 43 49
    88# 68 141 54
  
3.13      
 108 89 169 52 46
    81 71 99 41
  
6.25      
 90 82 188 70 59
    96 76 141 49
  
12.5       
 108 96 140 43 40
    68 49 75 37
  
25         
 100 89 177 59 50
    103 93 128 41
  
50         
 95 85 156 55 54
    85 82 135 53
  
100         
   
Positive Control 3-Methylcholanthrene 
5         
 33 5 255 256 244*
    29 3 249 288
    40# 7 226.5 187

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 80 78 39 30
    83# 90 58.5 24
    95 124 84 30
    83 106 68 27
Test Chemical Progesterone 
40         
 85# 89 43.5 17 22
    81 83 50 21
    93 77 82 29
  
50         
 95 70 82 29 27
    82# 90 73.5 30
    88 83 60 23
  
60         
 83 71 69 28 29
    93 59 83 30
    80 57 72 30
  
70         
 83# 29 88.5 36 38
    87 71 104 40
    75 56 88 39
Positive Control 3-Methylcholanthrene 
5         
 11 3 222 683 521*
    15 4 191 415
    13# 4 174 464

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 81 63 24 24
    96 107 64 22
    107 106 77 24
    92 106 76 28
Test Chemical Progesterone 
40         
 68# 77 49.5 24 29
    101# 74 100.5 33
    86 16 79 31
  
50         
 65 4 112 57 50*
    79 59 90 38
    75 64 125 56
  
60         
 62*
    75 14 153 68
    78 66 129 55
  
70         
 92*
    61 4 188 103
    64 7 156 81
  
80         
   
   
Positive Control 3-Methylcholanthrene 
5         
 37 8 319 286 299*
    21 3 233 364
    37 6 276 246

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92 97 66 24 20
    102 104 68 22
    81 91 50 21
    102 108 37 12
Test Chemical Progesterone 
40         
 83# 94 38 15 20
    74 81 41 18
    77 79 58 25
  
50         
 102 107 81 26 25
    95# 90 67.5 24
  
60         
 94 104 57 20 22
    83 63 51 21
    79 83 58 25
  
70         
 89 85 71 27 34*
    81 65 86 35
    102 107 83 27
    94 24 128 46
  
80         
 36*
    90 27 77 29
    94 36 92 33
    97 18 135 47
  
90         
   
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 62 43 627 339 310*
    67 52 562 280

Induced S9 Trial: 5 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 73 88 109 50 47
    128r 192 73 19
    77 112 103 44
    120r 182 80 22
Test Chemical Progesterone 
40         
 70 81 145 69 65
    55# 39 121.5 74
    75 74 117 52
  
50         
 57 72 162 95 79*
    60# 77 132 73
    80# 89 170 71
  
60         
 83 128 61 25 27
    87 101 101 39
    99 125 56 19
  
70         
 81 98 133 55 48
    104 124 143 46
    89 96 117 44
  
80         
 76# 90 125 55 47
    102 43 120 39
    126r 107 133 35
  
90         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 54 63 399 245 213*
    66 72 430 218
    94 83 492 175

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination