Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02745-0011-0000-2
Chemical Name:	Propyl gallate
CASRN:	121-79-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	099874
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	99	149	62	66
			73	79	166	76
			91	91	204	75
			109	131	170	52
Test Chemical	Propyl gallate	62.5	72	15	1051	485	469*
			71	19	968	452	469*
		125	73	12	617	280	278*
			74	11	615	276	278*
		250	73	7	248	114	113*
			69	7	230	112	113*
		500	50	3	193	128	132*
			63	4	256	135	132*
		1000

Positive Control	Methyl Methane Sulfonate	15	37	25	419	379	397*
			41	28	514	415	397*
	Methyl Methane Sulfonate	250	70	79	453	217	235*
			56	79	420	252	235*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	95	114	46	38
			77	98	70	30
			85	101	85	34
			108	106	131	40
Test Chemical	Propyl gallate	50	75	16	518	231	210*
			80	17	456	190	210*
		100	70	11	262	125	136*
			67	13	297	147	136*
		200	70	7	203	97	100*
			56	7	171	102	100*
		300

Positive Control	Methyl Methane Sulfonate	15	45	27	250	187	179*
			46	27	233	171	179*
	Methyl Methane Sulfonate	250	77	64	354	154	158*
			71	60	346	162	158*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	109	108	55	60
			75	99	166	73
			75	97	103	46
			64	95	127	66
Test Chemical	Propyl gallate	0.5	77	62	287	124	112*
			72	62	215	100	112*
		1	76	19	381	166	182*
			64	23	378	198	182*
		2	72	19	360	166	171*
			58	17	307	175	171*
		4	55	17	271	165	166*
			57	13	283	167	166*
		5	59	16	325	184	156*
			90	22	342	127	156*
Positive Control	Methyl Methane Sulfonate	15	21	18	279	439	432*
Positive Control			25	17	321	425	432*

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	111	122	56	51
			64	93	121	63
			86	105	102	40
			80	91	106	44
Test Chemical	Propyl gallate	25	54	17	698	431	425*
			65	20	810	419	425*
		50	42	16	685	542	508*
			51	18	727	475	508*
		75	63	16	813	428	438*
			48	11	640	448	438*
		100	45	11	690	517	455*
			60	12	711	393	455*
		125	60#	7	631	354	390*
			46	7	592	427	390*
Positive Control	Methyl Methane Sulfonate	15	19	18	371	645	640*
Positive Control			14	12	260	634	640*

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	99	168	72	65
			66	92	121	61
			49r	87	135	92
			82	109	153	62
Test Chemical	Propyl gallate	12.5	70	44	446	211	184*
			65	40	306	157	184*
		25	45	18	568	425
			82r	17	815	333
		50	62	17	446	240	278*
			52	14	491	317	278*
		75	63	8	673	358	334*
			57	9	527	309	334*
		100	31	8	287	312	313*
			38	8	360	314	313*
		125

Positive Control	Methyl Methane Sulfonate	15	21	11	256	406	426*
Positive Control			26	12	347	445	426*

Nonactivation Trial: 6 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	99	127	58	48
			99	110	114	38
			79#	86	117	50
			70	106	98	46
Test Chemical	Propyl gallate	12.5	83	18	625	251	249*
			73	17	542	247	249*
		25	93	14	284	102	108*
			91	15	312	115	108*
		50	52	8	226	146	138*
			54	9	210	131	138*
		75
		100

Positive Control	Methyl Methane Sulfonate	15	23	25	288	411	383*
Positive Control			27	26	285	354	383*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination