Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02747-0007-0000-9
Chemical Name:	Propylene
CASRN:	115-07-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	537921
Study Result:	Negative

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	78	88	106	46	49
			94#	103	148	53
			83	101	114	46
			82	108	126	51
Test Chemical	Propylene	2.5	80	78	117	49	47
			83	86	112	45	47
		5	83	90	91	36	44
			79	107	120	51	44
		10	72	84	76	35	35
			69	99	73	35	35
		20	83	93	73	29	30
			75	82	70	31	30
		30	87	96	128	49	45
			84	85	102	41	45
Positive Control	Methyl Methane Sulfonate	15	28	13	340	407	395*
Positive Control			24	19	272	383	395*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	93	98	43	15	23
			86	95	78	30
			75	112	76	34
			81	95	29	12
Test Chemical	Propylene	2.5	66	83	65	33	29
			87	93	65	25	29
		5	65	76	44	23	21
			65	78	40	20	21
		10	60	77	89	49	33
			64	81	33	17	33
		20	61	84	52	28	30
			53	81	49	31	30
		30	57	71	37	22	21
			60	77	36	20	21
Positive Control	Methyl Methane Sulfonate	15	19	14	137	240	222*
Positive Control			24	11	146	203	222*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	91	117	47	17	25
			89	90	65	24
			94	98	79	28
			86	96	79	31
Test Chemical	Propylene	2.5	77	59	39	17	21
			83	88	63	25	21
		5	80	92	43	18	19
			82	80	49	20	19
		10	88	86	73	28	18
			73	89	20	9	18
		20	86	85	76	29	27
			56	62	41	24	27
		30	76	77	49	21	22
			71	79	49	23	22
Positive Control	3-Methylcholanthrene	2.5	57	35	288	167	176*
Positive Control			61	36	338	185	176*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Air	0	90	96	43	16	17
			70	88	33	16
			93	114	50	18
			78	102	47	20
Test Chemical	Propylene	2.5	81	85	80	33	28
			85	94	60	24	28
		5	86	79	88	34	32*
			76	85	68	30	32*
		10	87	78	71	27	30*
			77	75	77	33	30*
		20	71	88	85	40	32*
			71	64	53	25	32*
		30	87	93	95	37	45*
			67	62	109	54	45*
Positive Control	3-Methylcholanthrene	2.5	105	117	66	21	19
Positive Control			81	113	43	18	19

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Air	0	69	106	37	18	21
			60	89	33	18
			70	103	54	26
			69	102	46	22
Test Chemical	Propylene	10	59	105	21	12	23
			57	91	58	34	23
		20	68	106	75	37	39*
			73	113	89	41	39*
		30	51	91	37	24	27
			55	103	50	30	27
		40	67	108	65	33	36*
			58	110	68	39	36*
		50	53	77	51	32	30
			73	118	63	29	30
Positive Control	3-Methylcholanthrene	2.5	40	40	244	206	229*
Positive Control			41	45	307	253	229*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination