Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02755-0004-0000-5
Chemical Name:	Pyrene
CASRN:	129-00-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	094614
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	50	98	19	13	13
			47r	85	11	8
			55	102	21	13
			44r	79	44	33
Test Chemical	Pyrene	42	55	91	32	20	19
			66	124	38	19	19
		52	57	108	43	25	19
			50	84	20	13	19
		66	43	72	35	27	23
			50	74	29	20	23
		82	43	64	14	11	10
			48	75	13	9	10
		102	47	76	30	21	21
			42	72	26	21	21
		128	47	72	29	21	18
			44	61	21	16	18
		160	44	71	17	13	12
			52	82	17	11	12
Positive Control	Ethyl Methane Sulfonate	500	27	33	429	526	523*
			32	42	445	468
			27	33	465	574

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	88	160	62	58
			86	98	165	64
			87#	104	168	64
			96#	109	120	42
Test Chemical	Pyrene	66	92	74	142	51	49
			96	79	124	43
			97	80	156	54
		82	88	60	135	51	58
			91	73	154	56
			87	64	172	66
		102	86	50	139	54	54
			81	75	120	50
			87	81	152	58
		128	87	75	133	51	44
			91	85	108	40
			106	101	127	40
		160	88	86	135	51	51
			81	48	142	58
			90	88	118	44
		200	89#	76	151.5	57	49
			93	52	125	45
			89	68	125	47
		250	91	75	171	62	63
			91	78	172	63
			87	78	167	64
Positive Control	Ethyl Methane Sulfonate	500	60	41	1158	647	602*
			64	45	1133	592
			70	45	1193	568

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	93	28	14	19
			70	96	36	17
			74	111	58	26
Test Chemical	Pyrene	5.9	58	37	182	105	117*
			54	30	212	130	117*
		6.6	53	29	161	102	113*
			47	26	172	123	113*
		7.3	61	23	237	129	137*
			51	21	221	145	137*
		8.1	38	13	259	226	185*
			37	12	158	144	185*
		9	34	8	33	32	157*
			27	7	228	281	157*
		10	15	1	7	16	22
			20	3	17	29	22
Positive Control	3-Methylcholanthrene	5	59	51	234	132	132*
			56	51	232	137
			54	59	208	128

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	107	125	42	41
			100	103	139	46
			93	87	95	34
			104	102	126	40
Test Chemical	Pyrene	2.8	93	92	135	48	57
			84	87	168	66	57
		3.5	86	88	194	76	69*
			88	91	167	63	69*
		4.4	85	82	160	63	58
			95	83	151	53	58
		4.9	92	71	175	64	68*
			83	64	180	72	68*
		6.1	81	35	253	104	93*
			89	40	219	82	93*
		7.7	83	15	311	126	127*
			82	18	313	128	127*
		9.6	64	6	209	108	113*
			68	7	237	117	113*
		12

Positive Control	3-Methylcholanthrene	5	83	63	468	189	200*
			75	57	461	206
			82	62	502	205

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination