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Study Information
Study Attribute Value
CEBS Accession Number: 002-02755-0004-0000-5
Chemical Name: Pyrene
CASRN: 129-00-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 094614
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 50 98 19 13 13
    47r 85 11 8
    55 102 21 13
    44r 79 44 33
Test Chemical Pyrene 
42         
 55 91 32 20 19
    66 124 38 19
  
52         
 57 108 43 25 19
    50 84 20 13
  
66         
 43 72 35 27 23
    50 74 29 20
  
82         
 43 64 14 11 10
    48 75 13 9
  
102         
 47 76 30 21 21
    42 72 26 21
  
128         
 47 72 29 21 18
    44 61 21 16
  
160         
 44 71 17 13 12
    52 82 17 11
Positive Control Ethyl Methane Sulfonate 
500         
 27 33 429 526 523*
    32 42 445 468
    27 33 465 574

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 88 160 62 58
    86 98 165 64
    87# 104 168 64
    96# 109 120 42
Test Chemical Pyrene 
66         
 92 74 142 51 49
    96 79 124 43
    97 80 156 54
  
82         
 88 60 135 51 58
    91 73 154 56
    87 64 172 66
  
102         
 86 50 139 54 54
    81 75 120 50
    87 81 152 58
  
128         
 87 75 133 51 44
    91 85 108 40
    106 101 127 40
  
160         
 88 86 135 51 51
    81 48 142 58
    90 88 118 44
  
200         
 89# 76 151.5 57 49
    93 52 125 45
    89 68 125 47
  
250         
 91 75 171 62 63
    91 78 172 63
    87 78 167 64
Positive Control Ethyl Methane Sulfonate 
500         
 60 41 1158 647 602*
    64 45 1133 592
    70 45 1193 568

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 67 93 28 14 19
    70 96 36 17
    74 111 58 26
Test Chemical Pyrene 
5.9       
 58 37 182 105 117*
    54 30 212 130
  
6.6       
 53 29 161 102 113*
    47 26 172 123
  
7.3       
 61 23 237 129 137*
    51 21 221 145
  
8.1       
 38 13 259 226 185*
    37 12 158 144
  
9         
 34 8 33 32 157*
    27 7 228 281
  
10         
 15 1 7 16 22
    20 3 17 29
Positive Control 3-Methylcholanthrene 
5         
 59 51 234 132 132*
    56 51 232 137
    54 59 208 128

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 100 107 125 42 41
    100 103 139 46
    93 87 95 34
    104 102 126 40
Test Chemical Pyrene 
2.8       
 93 92 135 48 57
    84 87 168 66
  
3.5       
 86 88 194 76 69*
    88 91 167 63
  
4.4       
 85 82 160 63 58
    95 83 151 53
  
4.9       
 92 71 175 64 68*
    83 64 180 72
  
6.1       
 81 35 253 104 93*
    89 40 219 82
  
7.7       
 83 15 311 126 127*
    82 18 313 128
  
9.6       
 64 6 209 108 113*
    68 7 237 117
  
12         
   
Positive Control 3-Methylcholanthrene 
5         
 83 63 468 189 200*
    75 57 461 206
    82 62 502 205

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination