Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02755-0005-0000-6
Chemical Name:	Pyrene
CASRN:	129-00-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	398036
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	105	107	52	16	21
			107	117	63	20
			92	81	84	30
			105	96	59	19
Test Chemical	Pyrene	7.8	91	72	68	25	23
			86	79	56	22	23
		15.6	85	82	74	29	21
			101	97	39	13	21
		31.3	91r	79	56	21
			127r	93	66	17
		62.5	77	77	30	13	18
			57	57	40	23	18
		125	66	46	52	26	23
			72#	66	40.5	19	23
		250	91	49	68	25	26
			77	44	62	27	26
Positive Control	Ethyl Methane Sulfonate	500	17	7	449	872	813*
			25#	12	606	824
			26	7	584	744

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	115	120	58	17	19
			95	108	53	19
			102	94	62	20
			98	78	63	21
Test Chemical	Pyrene	7.8	102	102	60	20	19
			105	124	57	18
			100	110	58	19
		15.6	97	70	60	21	20
			82	90	39	16
			104	81	76	24
		31.3	81#	82	57	24	22
			87	86	58	22
			104	81	65	21
		62.5	87	82	46	18	21
			93	92	66	24
			101	87	67	22
		125	92	10	98	35	30
			93	48	73	26
			90	50	80	30
Positive Control	Ethyl Methane Sulfonate	500	36#	15	861	794	811*
			34	13	919	892
			41	14	908	747

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	110	100	34	23
			98	82	60	20
			86	99	53	21
			86	108	47	18
Test Chemical	Pyrene	0.24	76	86	100	44	44*
			88	86	118	45	44*
		0.49	101	123	97	32	33
			96#	110	94.5	33	33
		0.98	75	85	83	37	38*
			109	85	130	40	38*
		1.95	70	68	60	28	36
			95	80	125	44	36
		3.9	60	37	110	61	53*
			78	51	104	44	53*
		7.8	56	10	227	136	123*
			69	14	227	110	123*
Positive Control	3-Methylcholanthrene	5	59	37	445	252	308*
			45	21	492	369
			55	22	499	303

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	86	67	27	24
			95	125	61	21
			97	94	72	25
			81	95	56	23
Test Chemical	Pyrene	0.25	80	129	72	30	32
			64	80	64	34	32
		0.5	72	84	75	35	39*
			77	110	102	44	39*
		1	79	75	110	46	45*
			86	83	115	44	45*
		2	99	67	101	34	38
			94	88	118	42	38
		4	88	49	160	61	55*
			71	37	107	50	55*
		6	77	24	108	47	39
			91	43	86	31	39
Positive Control	3-Methylcholanthrene	5	54	36	388	241	215*
			59	41	371	208
			59	36	343	194

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64#	96	73	38	47
			87	154	127	49
			52	51	82	52
			69	100	102	49
Test Chemical	Pyrene	1	58	81	100	58	60
			69	66	131	63	60
		2	49	52	138	93	80*
			60	67	120	67	80*
		4	65#	48	138	71	99*
			54	44	206	127	99*
		5	71	20	383	179	104*
			80#	58	184.5	77
			72	62	123	57
		6	65#	35	178.5	91	99*
			65	29	189	97
			65	12	212	108
		7	65	39	214	110	121*
			74	17	302	137
			50	24	175	116
		8	57	13	278	164	166*
			51	12	260	170
			51	11	251	165
Positive Control	3-Methylcholanthrene	5	34	3	340	332	347*
			15#	1	205.5	447
			21	2	162	261

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination