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Study Information
Study Attribute Value
CEBS Accession Number: 002-02767-0001-0000-5
Chemical Name: Reactive blue 19
CASRN: 2580-78-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 372640_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Weakly Positive 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 09/22/1988    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1050 380 0.362 7.60 26.0 0.000
Test Chemical Reactive blue 19 
5         
 50 1049 403 0.384 8.06 26.0 6.154 
16         
 50 1048 419 0.400 8.38 26.0 10.474 
50         
 50 1050 392 0.373 7.84 26.0 3.158 
160         
 50 1047 477 0.456 9.54 26.0 25.886
Positive Control Mitomycin-C 
 0.0E-4    
 50 1048 509 0.486 10.18 26.0 34.203
Mitomycin-C 
 0.005     
 10 209 268 1.282 26.80 26.0 254.319
Trend: 2.879        
Probability: 0.002        

Trial #: 2    Activation: No Activation    Date: 11/08/1988    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1039 402 0.387 8.04 26.0 0.000
Test Chemical Reactive blue 19 
50         
 50 1048 369 0.352 7.38 26.0 -8.997 
100         
 50 1046 412 0.394 8.24 26.0 1.802 
160         
 50 1046 396 0.379 7.92 26.0 -2.152 
300         
 50 1044 535 0.512 10.70 31.0 32.447
Positive Control Mitomycin-C 
 0.0E-4    
 50 1022 519 0.508 10.38 26.0 31.252
Mitomycin-C 
 0.005     
 10 206 263 1.277 26.30 26.0 229.973
Trend: 4.472        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 10/19/1989    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1031 398 0.386 7.96 26.0 0.000
Test Chemical Reactive blue 19 
100         
 50 1035 485 0.469 9.70 26.0 21.388 
160         
 50 1028 521 0.507 10.42 26.0 31.287 
300         
 50 1026 579 0.564 11.58 26.0 46.186
Positive Control Mitomycin-C 
 0.0E-4    
 50 1037 635 0.612 12.70 26.0 58.625
Mitomycin-C 
 0.005     
 10 210 278 1.324 27.80 26.0 242.927
Trend: 5.926        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/22/1988    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1049 396 0.378 7.92 26.0 0.000
Test Chemical Reactive blue 19 
16         
 50 1049 373 0.356 7.46 26.0 -5.808 
50         
 50 1050 435 0.414 8.70 26.0 9.744 
160         
 50 1045 499 0.478 9.98 26.0 26.492 
500         
 50 1044 447 0.428 8.94 26.0 13.419
Positive Control Cyclophosphamide 
 0.1       
 50 1046 518 0.495 10.36 26.0 31.183
Cyclophosphamide 
 0.6       
 10 210 173 0.824 17.30 26.0 118.226
Trend: 3.570        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 10/19/1989    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1046 392 0.375 7.84 26.0 0.000
Test Chemical Reactive blue 19 
100         
 50 1049 465 0.443 9.30 26.0 18.283 
160         
 50 1039 431 0.415 8.62 26.0 10.690 
300         
 50 1029 486 0.472 9.72 26.0 26.028
Positive Control Cyclophosphamide 
 0.1       
 50 1029 524 0.509 10.48 26.0 35.882
Cyclophosphamide 
 0.6       
 10 209 191 0.914 19.10 26.0 143.856
Trend: 2.988        
Probability: 0.001