Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-02769-0017-0000-4
Chemical Name: Reserpine
CASRN: 50-55-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 562201
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 93 98 76 27 24
    93 102 68 24
    64 84 54 28
    101 115 54 18
Test Chemical Reserpine 
 0.625     
 87 110 51 20 17
    70 92 31 15
  
1.25      
 75 94 45 20 24
    83 100 70 28
  
2.5       
 66 74 42 21 21
    66 82 40 20
  
5         
 99 125 72 24 20
    83 110 38 15
  
10         
 76 87 39 17 19
    86 96 55 21
Positive Control Methyl Methane Sulfonate 
15         
 40 25 144 122 117*
    48 30 163 112
Methyl Methane Sulfonate 
250         
 98 88 450 153 163*
    84 70 439 174

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 85 78 30 22
    100 101 71 24
    95 106 52 18
    95 108 46 16
Test Chemical Reserpine 
2         
 98 100 82 28 24
    93 111 53 19
  
4         
 88 92 86 33 33
    87 85 85 33
  
6         
 81 85 67 27 31
    92 91 94 34
  
8         
 79 89 64 27 33
    85 88 102 40
  
10         
 84 74 85 34 32
    84 68 78 31
Positive Control Methyl Methane Sulfonate 
15         
 47 33 113 80 79*
    51 35 120 78
Methyl Methane Sulfonate 
250         
 87 79 431 166 167*
    70 84 356 169

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 65 88 85 44 46
    76 86 114 50
    74 115 94 42
    59 112 83 47
Test Chemical Reserpine 
6         
 54 62 115 71 55
    67 80 77 38
  
7         
 63 81 115 61 59
    56 79 97 58
  
8         
 59 76 96 54 58
    62 56 116 63
  
9         
 57 72 99 58 60
    55 69 101 62
  
10         
 57 85 103 60 62
    66 92 128 64
Positive Control 3-Methylcholanthrene 
2.5       
 36 17 497 456 520*
    31 17 537 584

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 104 157 65 71
    84# 110 174 69
    78 104 192 82
    76 82 155 68
Test Chemical Reserpine 
6         
 61 34 229 125 126*
    76 84 288 127
  
7         
 63 64 326 173 161*
    72 79 324 149
  
8         
 59 66 249 141 145*
    65 74 288 148
  
9         
 74 68 221 100 99
    57# 51 168 99
  
10         
 57 60 184 107 95
    69 70 172 84
Positive Control 3-Methylcholanthrene 
2.5       
 62 48 552 298 308*
    61 45 580 318

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 112 103 130 39 48
    106 88 121 38
    87 108 165 63
    94 101 148 52
Test Chemical Reserpine 
6         
 107 93 163 51 47
    85 77 112 44
  
7         
 108 64 171 53 52
    93 68 141 51
  
8         
 96 68 174 60 55
    101 79 153 50
  
9         
 95 71 165 58 58
    85 74 148 58
  
10         
 90 70 99 37 34
    89 76 85 32
Positive Control 3-Methylcholanthrene 
2.5       
 58 40 656 380 375*
    58 36 642 369

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination