Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02769-0017-0000-4
Chemical Name:	Reserpine
CASRN:	50-55-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	562201
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	93	98	76	27	24
			93	102	68	24
			64	84	54	28
			101	115	54	18
Test Chemical	Reserpine	0.625	87	110	51	20	17
			70	92	31	15	17
		1.25	75	94	45	20	24
			83	100	70	28	24
		2.5	66	74	42	21	21
			66	82	40	20	21
		5	99	125	72	24	20
			83	110	38	15	20
		10	76	87	39	17	19
			86	96	55	21	19
Positive Control	Methyl Methane Sulfonate	15	40	25	144	122	117*
			48	30	163	112	117*
	Methyl Methane Sulfonate	250	98	88	450	153	163*
			84	70	439	174	163*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	85	78	30	22
			100	101	71	24
			95	106	52	18
			95	108	46	16
Test Chemical	Reserpine	2	98	100	82	28	24
			93	111	53	19	24
		4	88	92	86	33	33
			87	85	85	33	33
		6	81	85	67	27	31
			92	91	94	34	31
		8	79	89	64	27	33
			85	88	102	40	33
		10	84	74	85	34	32
			84	68	78	31	32
Positive Control	Methyl Methane Sulfonate	15	47	33	113	80	79*
			51	35	120	78	79*
	Methyl Methane Sulfonate	250	87	79	431	166	167*
			70	84	356	169	167*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	88	85	44	46
			76	86	114	50
			74	115	94	42
			59	112	83	47
Test Chemical	Reserpine	6	54	62	115	71	55
			67	80	77	38	55
		7	63	81	115	61	59
			56	79	97	58	59
		8	59	76	96	54	58
			62	56	116	63	58
		9	57	72	99	58	60
			55	69	101	62	60
		10	57	85	103	60	62
			66	92	128	64	62
Positive Control	3-Methylcholanthrene	2.5	36	17	497	456	520*
Positive Control			31	17	537	584	520*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	104	157	65	71
			84#	110	174	69
			78	104	192	82
			76	82	155	68
Test Chemical	Reserpine	6	61	34	229	125	126*
			76	84	288	127	126*
		7	63	64	326	173	161*
			72	79	324	149	161*
		8	59	66	249	141	145*
			65	74	288	148	145*
		9	74	68	221	100	99
			57#	51	168	99	99
		10	57	60	184	107	95
			69	70	172	84	95
Positive Control	3-Methylcholanthrene	2.5	62	48	552	298	308*
Positive Control			61	45	580	318	308*

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	112	103	130	39	48
			106	88	121	38
			87	108	165	63
			94	101	148	52
Test Chemical	Reserpine	6	107	93	163	51	47
			85	77	112	44	47
		7	108	64	171	53	52
			93	68	141	51	52
		8	96	68	174	60	55
			101	79	153	50	55
		9	95	71	165	58	58
			85	74	148	58	58
		10	90	70	99	37	34
			89	76	85	32	34
Positive Control	3-Methylcholanthrene	2.5	58	40	656	380	375*
Positive Control			58	36	642	369	375*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination