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Study Information
Study Attribute Value
CEBS Accession Number: 002-02779-0002-0000-9
Chemical Name: Rhodamine 6G
CASRN: 989-38-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 199566_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Negative 
Induced Rat Liver S9  4_S9  Test Failure 

Trial #:1   Activation: No Activation    Date: 08/13/1985    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Ethanol 
0         
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0
Test Chemical: Rhodamine 6G 
 0.196     
 200 6 0.030 3.0 0 0.000 0.0 6 0.030 3.0 0 0.000 0.0 
 0.59      
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0 
1.96      
 120 1 0.008 0.8 0 0.000 0.0 1 0.008 0.8 0 0.000 0.0 
5.9       
 50 5 0.100 8.0 0 0.000 0.0 5 0.100 8.0 0 0.000 0.0 
19.6       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 200 34 0.170 15.0 6 0.030 3.0 28 0.140 12.0 0 0.000 0.0
Mitomycin-C 
5         
 50 28 0.560 36.0 5 0.100 10.0 23 0.460 28.0 0 0.000 0.0
Trend: 0.441 0.000 0.441  
Probability: 0.330 0.000 0.330  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/01/1985    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Ethanol 
0         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
Test Chemical: Rhodamine 6G 
10         
 200 8 0.040 4.0 3 0.015 1.5 5 0.025 2.5 0 0.000 0.0 
15         
 200 16 0.080 6.0 5 0.025 2.5 11 0.055 4.5 0 0.000 0.0 
20         
 200 43 0.215 7.5 14 0.070 3.5 19 0.095 6.0 10 0.050 1.0 
25         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 10 84 8.400 100.0 0 0.000 0.0 4 0.400 20.0 80 8.000 8.0
Trend: 3.231 2.671 2.930  
Probability: 0.001 0.004 0.002  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 01/02/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Ethanol 
0         
 200 4 0.020 2.0 0 0.000 0.0 3 0.015 1.5 1 0.005 1.0
Test Chemical: Rhodamine 6G 
9.9       
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0 
19.8       
 200 91 0.455 17.0 22 0.110 9.0 29 0.145 8.5 40 0.200 4.0 
29.7       
 200 168 0.840 28.0 36 0.180 14.0 61 0.305 17.5 71 0.355 8.0
Positive Control: Cyclophosphamide 
10         
 50 59 1.180 42.0 15 0.300 24.0 14 0.280 20.0 30 0.600 3.0
Cyclophosphamide 
50         
 10 100 10.000 100.0 0 0.000 0.0 0 0.000 0.0 100 10.000 10.0
Trend: 8.843 6.945 6.350  
Probability: 0.000 0.000 0.000  


Trial #:3_S9   Activation: Induced Rat Liver S9    Date: 08/13/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Ethanol 
0         
 200 5 0.025 2.5 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: Rhodamine 6G 
1.96      
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0 
5.9       
 200 3 0.015 1.5 2 0.010 1.0 1 0.005 0.5 0 0.000 0.0 
19.6       
 200 5 0.025 2.5 0 0.000 0.0 5 0.025 2.5 0 0.000 0.0 
39.2       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 32 0.640 24.0 3 0.060 6.0 9 0.180 16.0 20 0.400 2.0
Trend: 0.041 -0.895 0.567  
Probability: 0.483 0.815 0.285  


Trial #:4_S9   Activation: Induced Rat Liver S9    Date: 09/24/1985    Harvest Time: 20 hour(s)   Trial Call: Test Failure   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Ethanol 
0         
 200 16 0.080 7.5 3 0.015 1.5 13 0.065 6.5 0 0.000 0.0
Test Chemical: Rhodamine 6G 
9.9       
 200 17 0.085 8.0 3 0.015 1.5 14 0.070 6.5 0 0.000 0.0 
14.9       
 200 45 0.225 13.5 3 0.015 1.0 32 0.160 12.5 10 0.050 1.0 
19.9       
 200 42 0.210 9.5 11 0.055 4.0 21 0.105 7.0 10 0.050 1.0 
29.9       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 10 89 8.900 100.0 1 0.100 10.0 8 0.800 20.0 80 8.000 8.0
Trend: 1.352 1.464 1.025  
Probability: 0.088 0.072 0.153