Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02779-0008-0000-5
Chemical Name:	Rhodamine 6G
CASRN:	989-38-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	039603
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	89	110	137	51	36
			83	88	81	32
			101	110	112	37
			94	92	69	24
Test Chemical	Rhodamine 6G	1.25	70	12	68	32	50
			52	9	108	69	50
		2.5	67	13	99	49	64*
			50	12	91	61
			59	13	146	83
		3.75	44	8	53	40	41
			49	8	78	53
			58	9	54	31
		5	64	11	97	51	72*
			46	5	133	96
			45	6	92	69
		7.5


Positive Control	Methyl Methane Sulfonate	5	92	83	663	239	233*
Positive Control			74	70	505	227	233*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	61	106	107	58	57
			48r	74	119	83
			37r	66	58	53
			52	94	85	55
Test Chemical	Rhodamine 6G	2	65	35	80	41	48
			49	23	78	53
			53	27	79	49
		3	52	20	129	82	78
			50	22	94	62
			45	20	123	91
		4	51	15	153	100	90*
			46	15	134	96
			53	19	116	73
		5	47	18	93	66	79
			63	10	207	110
			63	18	113	60
		6					129*
			61	8	207	113
			37	5	158	144
		8


Positive Control	Methyl Methane Sulfonate	5	26	24	351	453	403*
Positive Control			32	32	340	352	403*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	83	103	101	40	40
			78#	78	108	46
			92	138	106	38
			74	82	82	37
Test Chemical	Rhodamine 6G	2	67#	44	81	40	38
			62	38	64	34
			56	32	68	41
		3	58	44	102	59	57
			54	25	86	53
			40	27	70	59
		4	47#	17	55	39	48
			61#	31	73	40
			47	26	90	65
		5	49	12	108	73	78*
			53	11	151	95
			52	15	105	67
		6	48	11	65	45	93*
			51	10	122	79
			49	7	225	154
		8	60	11	129	72	134*
			37	9	217	195
			38	7	153	135
		10


Positive Control	Methyl Methane Sulfonate	5	68	55	542	268	260*
			77#	61	537	234
			63	49	522	277

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	106	87	281	89	65
			81	100	138	57
			79	119	118	50
			78	94	154	66
Test Chemical	Rhodamine 6G	2.5	81	39	94	39	41
			97	61	115	40
			64#	46	86	45
		5	73#	36	116	53	50
			62	24	87	47
			76	32	113	50
		7.5	81	28	132	54	53
			78	25	111	48
			76	18	128	56
		10	56	16	91	54	50
			55	18	74	45
			59	17	90	51
		15

		20


Positive Control	3-Methylcholanthrene	2.5	49#	18	683	463	440*
			61	21	968	532
			93#	35	909	326

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination