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Study Information
Study Attribute Value
CEBS Accession Number: 002-02779-0008-0000-5
Chemical Name: Rhodamine 6G
CASRN: 989-38-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 039603
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 89 110 137 51 36
    83 88 81 32
    101 110 112 37
    94 92 69 24
Test Chemical Rhodamine 6G 
1.25      
 70 12 68 32 50
    52 9 108 69
  
2.5       
 67 13 99 49 64*
    50 12 91 61
    59 13 146 83
  
3.75      
 44 8 53 40 41
    49 8 78 53
    58 9 54 31
  
5         
 64 11 97 51 72*
    46 5 133 96
    45 6 92 69
  
7.5       
   
   
Positive Control Methyl Methane Sulfonate 
5         
 92 83 663 239 233*
    74 70 505 227

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 61 106 107 58 57
    48r 74 119 83
    37r 66 58 53
    52 94 85 55
Test Chemical Rhodamine 6G 
2         
 65 35 80 41 48
    49 23 78 53
    53 27 79 49
  
3         
 52 20 129 82 78
    50 22 94 62
    45 20 123 91
  
4         
 51 15 153 100 90*
    46 15 134 96
    53 19 116 73
  
5         
 47 18 93 66 79
    63 10 207 110
    63 18 113 60
  
6         
 129*
    61 8 207 113
    37 5 158 144
  
8         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 26 24 351 453 403*
    32 32 340 352

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 83 103 101 40 40
    78# 78 108 46
    92 138 106 38
    74 82 82 37
Test Chemical Rhodamine 6G 
2         
 67# 44 81 40 38
    62 38 64 34
    56 32 68 41
  
3         
 58 44 102 59 57
    54 25 86 53
    40 27 70 59
  
4         
 47# 17 55 39 48
    61# 31 73 40
    47 26 90 65
  
5         
 49 12 108 73 78*
    53 11 151 95
    52 15 105 67
  
6         
 48 11 65 45 93*
    51 10 122 79
    49 7 225 154
  
8         
 60 11 129 72 134*
    37 9 217 195
    38 7 153 135
  
10         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 68 55 542 268 260*
    77# 61 537 234
    63 49 522 277

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 106 87 281 89 65
    81 100 138 57
    79 119 118 50
    78 94 154 66
Test Chemical Rhodamine 6G 
2.5       
 81 39 94 39 41
    97 61 115 40
    64# 46 86 45
  
5         
 73# 36 116 53 50
    62 24 87 47
    76 32 113 50
  
7.5       
 81 28 132 54 53
    78 25 111 48
    76 18 128 56
  
10         
 56 16 91 54 50
    55 18 74 45
    59 17 90 51
  
15         
   
  
20         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 49# 18 683 463 440*
    61 21 968 532
    93# 35 909 326

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination