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Study Information
Study Attribute Value
CEBS Accession Number: 002-02784-0002-0000-5
Chemical Name: Rotenone
CASRN: 83-79-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 195015_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Questionable 

Trial #:1   Activation: No Activation    Date: 03/07/1985    Harvest Time: 20.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 2.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Acetone 
0         
 100 5 0.050 4.0 4 0.040 3.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Rotenone 
25         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
75         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
100         
 100 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.05      
 25 48 1.920 72.0 18 0.720 52.0 17 0.680 48.0 13 0.520 16.0
Mitomycin-C 
 0.08      
 10 27 2.700 80.0 14 1.400 60.0 13 1.300 60.0 0 0.000 0.0
Trend: -0.296 -0.985 0.111  
Probability: 0.616 0.838 0.456  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 02/20/1985    Harvest Time: 21.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Acetone 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Rotenone 
100         
 100 9 0.090 5.0 1 0.010 1.0 8 0.080 5.0 0 0.000 0.0 
150         
 100 7 0.070 6.0 2 0.020 2.0 5 0.050 4.0 0 0.000 0.0 
200         
 100 8 0.080 7.0 1 0.010 1.0 5 0.050 4.0 2 0.020 2.0 
250         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 100 16 0.160 13.0 8 0.080 7.0 8 0.080 6.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 50 28 0.560 26.0 2 0.040 4.0 16 0.320 24.0 10 0.200 2.0
Trend: 1.297 -0.320 0.937  
Probability: 0.097 0.626 0.174  


Trial #:2   Activation: No Activation    Date: 02/20/1985    Harvest Time: 21.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 9 0.090 5.0 1 0.010 1.0 8 0.080 5.0 0 0.000 0.0
Acetone 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Rotenone 
10         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
25         
 100 11 0.110 10.0 5 0.050 5.0 6 0.060 5.0 0 0.000 0.0 
50         
 100 3 0.030 2.0 0 0.000 0.0 3 0.030 2.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.04      
 100 22 0.220 14.0 6 0.060 6.0 16 0.160 10.0 0 0.000 0.0
Mitomycin-C 
 0.0625    
 25 20 0.800 32.0 4 0.160 12.0 5 0.200 16.0 11 0.440 8.0
Trend: 1.476 0.655 1.390  
Probability: 0.070 0.256 0.082