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Study Information
Study Attribute Value
CEBS Accession Number: 002-02784-0015-0000-9
Chemical Name: Rotenone
CASRN: 83-79-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 542524
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 88 106 153 58 59
    86 105 139 54
    59 89 117 66
Test Chemical Rotenone 
 0.5       
 65# 22 339 174 208*
    55# 20 400 241
  
1         
 58 14 423 242 323*
    53 15 637 403
  
2         
 31 6 1946 2104 1310*
    49# 7 753 516
  
4         
 17 3 1342 2631 3142*
    13# 2 1397 3652
  
8         
   
Positive Control Methyl Methane Sulfonate 
15         
 38 32 263 231 236*
    38# 31 274 240

Nonactivation Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 75# 86 201 90 80
    75# 72 169.5 76
    76 121 169 74
    72# 121 174 81
Test Chemical Rotenone 
2.5       
 79# 22 84 35
  
3.5       
 72 29 70 32 23
    74# 18 28.5 13
  
4.5       
 68# 26 66 32 27
    76# 26 49.5 22
  
5.5       
 73# 16 55.5 25 30
    78# 18 81 35
  
6.5       
 70# 19 42 20
Positive Control Methyl Methane Sulfonate 
15         
 59# 70 378 213 213*

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 71 91 125 59 71
    77 103 141 61
    72 97 186 87
    91 109 209 77
Test Chemical Rotenone 
 0.25      
 61 24 209 115 116*
    67 26 236 117
  
 0.5       
 66 37 182 92 119*
    50 28 218 146
  
1         
 74 23 411 184 238*
    56 17 492 292
  
2         
 50 16 443 298 268*
    58 18 412 238
  
4         
  
8         
   
Positive Control Methyl Methane Sulfonate 
15         
 35 31 159 151 191*
    35 25 242 232

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination