Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02784-0015-0000-9
Chemical Name:	Rotenone
CASRN:	83-79-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	542524
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	88	106	153	58	59
			86	105	139	54
			59	89	117	66
Test Chemical	Rotenone	0.5	65#	22	339	174	208*
			55#	20	400	241	208*
		1	58	14	423	242	323*
			53	15	637	403	323*
		2	31	6	1946	2104	1310*
			49#	7	753	516	1310*
		4	17	3	1342	2631	3142*
			13#	2	1397	3652	3142*
		8

Positive Control	Methyl Methane Sulfonate	15	38	32	263	231	236*
Positive Control			38#	31	274	240	236*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	75#	86	201	90	80
			75#	72	169.5	76
			76	121	169	74
			72#	121	174	81
Test Chemical	Rotenone	2.5	79#	22	84	35
		3.5	72	29	70	32	23
			74#	18	28.5	13	23
		4.5	68#	26	66	32	27
			76#	26	49.5	22	27
		5.5	73#	16	55.5	25	30
			78#	18	81	35	30
		6.5	70#	19	42	20
Positive Control	Methyl Methane Sulfonate	15	59#	70	378	213	213*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	71	91	125	59	71
			77	103	141	61
			72	97	186	87
			91	109	209	77
Test Chemical	Rotenone	0.25	61	24	209	115	116*
			67	26	236	117	116*
		0.5	66	37	182	92	119*
			50	28	218	146	119*
		1	74	23	411	184	238*
			56	17	492	292	238*
		2	50	16	443	298	268*
			58	18	412	238	268*
		4
		8

Positive Control	Methyl Methane Sulfonate	15	35	31	159	151	191*
Positive Control			35	25	242	232	191*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination