Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02785-0009-0000-3
Chemical Name:	Roxarsone
CASRN:	121-19-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	941114
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83#	99	74	30	33
			78	106	77	33
			75	89	91	40
			70	106	57	27
Test Chemical	Roxarsone	125	57	99	107	63	42
			102	150	74	24
			68	96	76	37
		250	67	102	70	35	30
			58	76	53	30
			73	113	53	24
		500	83	111	73	29	25
			71	101	48	23
			78	79	56	24
		750	67	36	87	43	35
			88	76	89	34
			86	91	76	29
		1000	85	40	99	39	34
			91#	47	86	32
			72	56	67	31
		1500


Positive Control	Methyl Methane Sulfonate	5	55	54	424	259	211*
			77	56	429	185
			78	59	442	188

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	103	72	27	26
			83	95	66	26
			76	103	52	23
			94	99	74	26
Test Chemical	Roxarsone	400	80	77	72	30	35
			70	82	82	39
			64	85	66	35
		500	74	62	59	26	26
			76	70	54	24
			89	76	71	26
		600	56	45	47	28	27
			70	59	45	21
			72	65	69	32
		800	56	28	60	36	35
			70	32	68	32
			58	29	63	36
		1000	38#	11	62	54	45*
			54	16	72	45
			67	29	75	37
		1200	60#	12	88	49	74*
			35	7	80	77
			38	7	110	96
		1500


Positive Control	Methyl Methane Sulfonate	5	28	19	253	305	260*
			40	23	257	217
			33	20	252	257

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	128	61	23	30
			64	86	69	36
			72	104	67	31
			67	82	64	32
Test Chemical	Roxarsone	400	75	61	95	42	36
			61	73	45	25
			55	66	66	40
		500	61	47	73	40	41
			61	59	84	46
			46	54	53	38
		600	52	52	62	40	44
			58	47	56	32
			58	30	102	59
		800	66	44	77	39	48*
			47	25	76	54
			56	32	87	52
		1000	73	22	140	64	71*
			63	19	132	69
			56	10	136	81
		1200


Positive Control	Methyl Methane Sulfonate	5	34	40	316	313	304*
			24	29	265	373
			39	47	265	226

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	103	58	30	28
			69	84	40	19
			80	119	67	28
			60	94	62	34
Test Chemical	Roxarsone	125	26	54	70	90	63*
			52	77	68	44
			44	80	75	57
		250	46	71	82	59	55*
			38	37	59	52
			35	49	57	54
		500	35	41	104	100	75*
			49	65	66	45
			43	41	101	79
		750	66	38	135	68	85*
			44	19	88	67
			34	12	121	119
		1000


Positive Control	3-Methylcholanthrene	2.5	27	25	408	501	642*
			25	30	388	521
			14	14	389	905

Induced S9 Trial: 2 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	102	81	184	60	37
			117	116	101	29
			129r	94	137	35
			107	102	68	21
Test Chemical	Roxarsone	500	94	55	83	29	35
			99	63	108	36
			67	48	78	39
		700	116	60	96	28	26
			121r	46	95	26
			91	66	69	25
		800	90	39	115	42	49
			38	18	71	63
			104	53	133	43
		900	87	18	159	61	47
			106	26	146	46
			91	46	90	33
		1000	135r	62	130	32	28
			114	53	96	28
			112	58	94	28
		1250


Positive Control	3-Methylcholanthrene	2.5	121r	57	402	111	112*
			126r	59	497	132
			109	75	363	112

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination