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Study Information
Study Attribute Value
CEBS Accession Number: 002-02792-0001-0000-3
Chemical Name: Safrole
CASRN: 94-59-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 292863_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #: 1    Activation: No Activation    Date: 11/23/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1046 505 0.483 10.10 26.0 -8.357
Dimethyl Sulfoxide 
0         
 50 1044 550 0.527 11.00 26.0 0.000
Test Chemical Safrole 
1.6       
 50 1051 575 0.547 11.50 26.0 3.849 
5         
 50 1044 631 0.604 12.62 26.0 14.727 
10         
 50 1048 588 0.561 11.76 26.0 6.501 
25         
 50 1040 598 0.575 11.96 26.0 9.145 
50         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.01      
 50 1049 2278 2.172 45.56 26.0 312.208
Trend: 1.627        
Probability: 0.052        

Trial #: 2    Activation: No Activation    Date: 12/13/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1031 382 0.371 7.64 26.0 0.000
Negative (Not Specified) 
0         
 50 1035 349 0.337 6.98 26.0 -8.992
Test Chemical Safrole 
10         
 50 1046 422 0.403 8.44 26.0 8.887 
30         
 50 1043 523 0.501 10.46 28.0 35.336 
50         
 50 1041 447 0.429 8.94 28.0 15.892
Positive Control Mitomycin-C 
 0.01      
 50 1038 2080 2.004 41.60 26.0 440.831
Trend: 3.476        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/23/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 458 0.440 9.16 26.0 0.000
Negative (Not Specified) 
0         
 50 1046 501 0.479 10.02 26.0 8.761
Test Chemical Safrole 
 0.5       
 50 1046 557 0.533 11.14 26.0 20.918 
1.6       
 50 1037 579 0.558 11.58 26.0 26.785 
5         
 50 1045 574 0.549 11.48 26.0 24.728 
16         
 50 1046 534 0.511 10.68 26.0 15.925 
25         
 50 1048 625 0.596 12.50 26.0 35.421 
50         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
2         
 50 1045 2078 1.989 41.56 26.0 351.541
Trend: 3.507        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 12/13/1982    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1036 418 0.403 8.36 26.0 0.000
Negative (Not Specified) 
0         
 50 1043 378 0.362 7.56 26.0 -10.176
Test Chemical Safrole 
10         
 50 1045 445 0.426 8.90 26.0 5.542 
30         
 50 1048 468 0.447 9.36 26.0 10.680 
50         
 50 1047 493 0.471 9.86 28.0 16.703 
75         
 50 1044 515 0.493 10.30 28.0 22.262
Positive Control Cyclophosphamide 
2         
 50 1043 1900 1.822 38.00 26.0 351.495
Trend: 3.362        
Probability: 0.000