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Study Information
Study Attribute Value
CEBS Accession Number: 002-02792-0002-0000-4
Chemical Name: Safrole
CASRN: 94-59-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 292863_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Questionable 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #:1   Activation: No Activation    Date: 12/13/1982    Harvest Time: 12.5 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Safrole 
40         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
50         
 100 7 0.070 7.0 3 0.030 3.0 4 0.040 4.0 0 0.000 0.0 
75         
 100 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 1 0.010 1.0 
100         
 100 6 0.060 5.0 4 0.040 4.0 1 0.010 1.0 1 0.010 1.0
Positive Control: Mitomycin-C 
 0.5       
 100 154 1.540 73.0 44 0.440 34.0 69 0.690 48.0 41 0.410 32.0
Trend: 1.354 1.730 -0.209  
Probability: 0.088 0.042 0.583  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 12/13/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Safrole 
30         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
40         
 100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 
50         
 100 12 0.120 8.0 5 0.050 5.0 6 0.060 3.0 1 0.010 1.0 
75         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 54 0.540 39.0 16 0.160 13.0 29 0.290 23.0 9 0.090 8.0
Trend: 2.208 1.662 1.426  
Probability: 0.014 0.048 0.077  


Trial #:2   Activation: No Activation    Date: 11/17/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Negative (Not Specified) 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Safrole 
 0.16      
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
 0.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1.6       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5         
 100 3 0.030 3.0 0 0.000 0.0 2 0.020 2.0 1 0.010 1.0 
16         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
50         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
160         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.5       
 100 53 0.530 36.0 19 0.190 14.0 22 0.220 22.0 12 0.120 11.0
Mitomycin-C 
 0.5       
 100 52 0.520 34.0 18 0.180 16.0 22 0.220 17.0 12 0.120 12.0
Trend: 2.181 -1.000 2.660  
Probability: 0.015 0.841 0.004  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 11/17/1982    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 1 0.010 1.0
Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Safrole 
 0.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1.6       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
5         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
16         
 100 3 0.030 3.0 3 0.030 3.0 0 0.000 0.0 0 0.000 0.0 
50         
 100 5 0.050 4.0 3 0.030 3.0 2 0.020 2.0 0 0.000 0.0 
160         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 97 0.970 52.0 39 0.390 30.0 29 0.290 22.0 29 0.290 21.0
Trend: 1.305 1.458 2.069  
Probability: 0.096 0.072 0.019