Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02792-0006-0000-8
Chemical Name:	Safrole
CASRN:	94-59-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	402751
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	109	46	16	16
			101	91	48	16
			100	103	58	19
			95	97	42	15
Test Chemical	Safrole	1.56	90	92	41	15	15
			97	115	41	14	15
		3.13	89	79	40	15	21
			81	88	68	28	21
		6.25	81	103	39	16	16
			78#	91	39	17	16
		12.5	95	83	67	24	19
			106	93	49	15	19
		25	77	70	50	22	23
			82	68	57	23	23
		50	94	52	52	18	26
			81	42	84	34	26
Positive Control	Ethyl Methane Sulfonate	500	39	21	998	853	793*
			49	20	1170	796
			55#	28	1206	729

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	115	56	26	27
			94	101	65	23
			82	92	82	34
			86	92	70	27
Test Chemical	Safrole	6.25	123r	125	50	14	19
			107#	130	28.5	9
			96#	93	82.5	29
		12.5	81	92	55	23	23
			100	91	60	20
			99	107	75	25
		25	99	43	62	21	26
			73	53	68	31
			87	71	70	27
		40	86	39	61	24	20
			88	31	59	22
			79#	61	36	15
		50	60	29	43	24	31
			61#	44	55.5	30
			84#	43	98	39
		60	69#	19	84	41	37
			87	38	78	30
			76	37	96	42
		80


Positive Control	Ethyl Methane Sulfonate	500	18#	10	694	1268	1138*
			34	23	814	798
			14	9	580	1349

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	88	62	21	20
			111#	117	78	23
			94	103	45	16
			100	92	59	20
Test Chemical	Safrole	6.25	84	94	73	29	26
			95	65	75	26
			99	80	66	22
		12.5	94	56	65	23	20
			103	79	72	23
			94	76	36	13
		25	88	44	78	29	22
			97	56	51	17
			99	47	58	20
		50	98	24	49	17	22
			86	35	83	32
			78#	38	37.5	16
		75	60	3	93	52	35

			94#	17	54	19
		100


Positive Control	Ethyl Methane Sulfonate	500	8r	1	330	1375	846*
			24	7	646	916
			29#	7	663	775

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	126	66	25	29
			80	91	64	27
			99	85	81	27
			88#	98	93	35
Test Chemical	Safrole	1.56	91	43	76	28	33
			81	58	93	38	33
		3.13	89	46	81	30	36
			100	42	128	43	36
		6.25	88	33	129	49	61*
			56	28	125	74	61*
		12.5	79	29	140	59	46
			99	42	95	32	46
		25	104	34	189	61	74*
			54	18	143	88	74*
		50
			88	34	119	45
		100

Positive Control	3-Methylcholanthrene	5	35	11	451	425	327*
			35	14	349	334
			58	26	386	221

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	74	104	210	95	90
			79	96	257	109
			64	86	159	83
			86	114	182	71
Test Chemical	Safrole	3.13	63	44	232	122	104
			76	50	218	96
			75	55	213	94
		6.25	67#	49	228	113	131*
			73	55	275	125
			64	50	299	155
		12.5	61	45	293	161	212*
			58	32	385	219
			59	31	455	256
		25	51	31	334	220	172*
			82	41	435	177
			102	44	366	120
		50	51	21	358	235	228*
			56	18	454	269
			72	18	480	223
			78	28	426	183
Positive Control	3-Methylcholanthrene	5					505*
			14	3	207	499
			12	2	189	511

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	46r	63	119	86	91
			58	84	170	98
			89	107	200	75
			64	110	191	100
Test Chemical	Safrole	3.13	45	60	141	105	86
			55	79	104	63
			57	57	153	90
		6.25	52	65	114	74	99
			52	34	190	122
			50	65	153	102
		12.5	38	14	190	168	122
			34	18	84	83
			40	28	136	114
		25	49	17	335	230	190*
			57	19	299	176
			61#	20	298.5	163
		50	81	13	543	223	265*
			69	7	584	282
			82	10	697	284
			69	11	555	270
Positive Control	3-Methylcholanthrene	5					656*
			17	1	328	656
			7r	1	142.5	712

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination