Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02792-0007-0000-9
Chemical Name:	Safrole
CASRN:	94-59-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	421768
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	85	99	55	22	21
			85#	94	46	18
			84	105	55	22
			91	102	63	23
Test Chemical	Safrole	3.13	70	65	60	29	31
			55#	63	57	34
			63	67	55	29
		6.25	76	72	47	21	28
			68	86	50	24
			60	63	72	40
		12.5	66	70	64	32	22
			59	68	29	16
			54	67	29	18
		25	82	76	81	33	32
			71	74	74	35
			77	67	64	28
		50	71	38	40	19	25
			84	48	62	25
			66	54	62	31
		100


Positive Control	Ethyl Methane Sulfonate	250	54	37	903	556	437*
			69#	45	862	414
			66#	57	670	341

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	101	103	92	30	28
Vehicle Control			99#	97	78	26	28
Test Chemical	Safrole	10	92#	85	54	20	31
			91#	82	115.5	42
			89#	84	85.5	32
		20	85	85	65	26	22
			82	76	47	19	22
		40	88#	54	75	28	27
			85#	64	45	18
			83	59	84	34
		50	92#	67	88.5	32	31
			87#	65	77	30	31
		60	79#	44	75	32	31
			100#	40	91	30	31
		80
			87#	15	138	53

		100


Positive Control	Methyl Methane Sulfonate	10	60	33	601	337	337*
Positive Control	Methyl Methane Sulfonate	250	73	65	773	351	351*

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	65	100	58	30	31
			95	122	85	30
			67	85	78	39
			63	93	48	25
Test Chemical	Safrole	30	73	92	64	29	25
			79	69	61	26
			68	78	39	19
		40	83#	103	64	26	28
			66	81	66	33
			84#	83	65	26
		50	73	84	77	35	36
			55	83	73	45
			84	69	68	27
		60	64	83	81	42	37
			85	62	91	36
			105#	60	105	33
		80	75	62	67	30	42
			63	33	101	54	42
		100

			53	33	63	39
		150


Positive Control	Methyl Methane Sulfonate	5	52	63	387	248	190*
			89	98	424	159
			65	96	316	163

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	69	86	122	59	55
			86	98	139	54
			98	103	172	59
			103	112	150	48
Test Chemical	Safrole	10	83	99	236	95
		20	94	75	222	79	83*
			87#	82	193	74
			105	73	302	95
		40	101	93	197	65	69
			75#	73	138	62
			76	69	182	80
		60	81	45	201	83	76
			84	75	185	74
			86	80	182	70
		80	99	60	290	98	97*
			101	66	298	99
			89	60	252	94
		100	84	47	188	74	95*
			85	35	253	99
			80	41	269	113
		120	80	23	293	122	112*
			85	34	260	102	112*
Positive Control	3-Methylcholanthrene	3.5	89	76	534	199	220*
			74	41	535	240
			88	80	580	220

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	109	77	113	35	34
			118	139	100	28
			111	85	130	39
			166r	181	100	20
Test Chemical	Safrole	0.02	85	32	164	64	62*
			160r	50	162	34
			85	31	150	59
		0.04	104	24	166	53	63*
			98	29	182	62
			96	26	208	72
		0.06					51
			108	28	166	51
			109#	23	163	50
		0.08	159r	21	367	77	69*
			110	25	240	73
			106#	19	207	65
		0.1	92	8	173	63	63*
			111	13	191	57
			114	16	230	67
		0.125


Positive Control	3-Methylcholanthrene	2.5	122r	77	524	143
Positive Control			123r	59	536	145

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	82	101	106	43	48
			63	80	120	64
			80	99	111	46
			91	120	100	37
Test Chemical	Safrole	0.02	88	65	109	41	46
			69	58	105	51	46
		0.04	82	47	121	49	52
			81	53	133	55	52
		0.06	76	43	113	49	54
			77	33	136	59
			94#	50	152	54
		0.08	112	48	168	50	55
			89	38	135	51
			69	33	131	63
		0.1	87	11	129	49	51
			91	38	130	47
			86	12	142	55
		0.125
			73	10	125	57

		0.15


Positive Control	3-Methylcholanthrene	2.5	90	77	480	178	163*
Positive Control			89	94	394	148	163*

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	72	98	90	42	61
			82	106	143	58
			54	84	133	82
			75	113	143	64
Test Chemical	Safrole	20	53	64	79	49	35
			77	78	71	31
			73	79	56	26
		40	63	68	79	42	39
			52	79	54	35
			60	75	73	41
		60	22	19	134	208	108*
			49	48	93	64
			62#	55	99	53
		80	58#	44	90	52	137*
			14	12	107	249
			14	17	46	110
		100	49	23	133	91	72
			41	37	107	88
			46	38	50	36
		125					91
			46	29	150	109
			44	10	96	73
		150


Positive Control	3-Methylcholanthrene	2.5	81	56	551	226	243*
			91	78	623	229
			59#	49	484	274

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	84	86	197	78	83
			99	109	216	73
			127r	121	324	85
			99	104	288	97
Test Chemical	Safrole	20	56	49	212	127	140*
			84	68	310	124
			73	50	370	169
		40	59	22	457	257	231*
			74	45	444	200
			62	26	434	235
		60	66	20	453	230	243*
			53	22	405	253
			63	26	462	245
		80	70	22	620	296	258*
			68	24	482	236
			85	22	612	241
		100	78	22	513	221	253*
			66	17	577	292
			88	64	651	246
		125	60	15	383	213	290*
			49	8	605	414
			73	22	530	243
		150


Positive Control	3-Methylcholanthrene	2.5					611*
			50	25	769	518
			29	6	616	704

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination